lab 2

Question 1

Techniques for purification procedures are often based on principles like

Answer 1

molecular size, electrostatic charge and solubility

Question 2

With the development of affinity chromatography, it is possible to

Answer 2

achieve a high degree of purification in a single step

Question 3

Affinity chromatography is based on

Answer 3

the ability of protein molecules to bind specifically and reversibly to appropriate ligands attached to insoluble supports

Question 4

Ligands may be

Answer 4

competitive inhibitors, coenzymes, substrates, or their analogs

Question 5

Colourimetric protein assays( such as _____, _____, and _____) are commonly used to measure

Answer 5

lowry, microbiuret, and bradford

the concentration of protein solutions because of their sensitivity and accuracy

Question 6

The bradford method is preferred because

Answer 6

it is rapid and is not affected by most non-protein components

Question 7

proteins and nucleic acids absorb in the UV range (_____) and show a peak at _____ for proteins and ______ for nucleic acids
The 280 peak for proteins in because of ______,_____ and _______

Answer 7

200-330
280
260
trptophan, tyrosine, and phenylalanine

Question 8

The absorbance at 280 can be used to measure

Answer 8

The concentration of crude protein solutions

Question 9

Absorbances at 215 nm provide what

but _____ will also absorb UV light

Answer 9

a very sensitive detection and measurement method for all proteins especially those with little or no trptophan
- the peptide bond