Lab 1: Oxygen Consumption - VO2 Flashcards
Charles’ Law
The volume of gas increases proportionately to an increase in temperature and vice versa (i.e., if the pressure is constant then a doubling of temperature will result in a doubling of volume)
Boyle’s Law
An increase in pressure causes an inversely proportionate decrease in volume and vice versa (i.e., if temperature is constant, then a doubling of pressure will cause a halving of volume).
Ideal Gas Law
PV = nRT
To compare measures of volume taken under different environmental conditions (i.e., comparing testing done at sea level in Vancouver versus at Everest base camp which has half the air pressure to that of sea level), there is a need for a standardized set of conditions, which are defined by BTPS
BTPS = body temperature and ambient pressure, saturated
To compare measures of volume taken under different environmental conditions (i.e., comparing testing done at sea level in Vancouver versus at Everest base camp, which has half the air pressure of sea level), there is a need for a standardized set of conditions, which are defined by STPD.
STPD = standard temperature and pressure, dry
Calculation of oxygen consumption (V̇O2)
STPD Correction Factor
What does Open-Circuit Spirometry measure?
What is The Haldane Transformation?
- At rest, expired gases contain more carbon dioxide (range~ 2.5-5.0%), and less oxygen (15.0-18.5%), than atmospheric air.
- This results in the volume of expired air (V̇E) being unequal to the inspired volume (V̇I)
The Haldene Transformation
Nitrogen
Nitrogen sees minimal (if any) change between inspired and expired air.
This is becasue N is a metabolically inert gas, meaning it serves no role in animal cellular metabolism.
The Haldene Transformation Equation
What is Steady state?
Steady state (or steady rate) reflects a balance between energy required by the working muscle and the ATP produced primarily through aerobic (oxygen using) metabolism
