Lab 1 Flashcards
What are indications to perform a bone marrow aspirate?
Unexplained thrombocytopenia, non-regenerative anemia, neutropenia, pancytopenia
Atypical cells peripheral blood
Staging neoplaisa,
Iron store in dog
Diagnosis infectious disease, histo, ehrlichiosis, cytauzoonosis and leishmaniasis
What are contradictions for bone marrow aspirate?
None
Low risk of trauma
Where can you take a bone marrow sample?
Greater tubercle of humerus
Trochanteric fossa of femur
Iliac crest of the pelvis
What supplies do you need for a bone marrow aspirate?
Scrub, alcohol, gauze, clippers
Sterile gloves
Bone marrow needle 18-13g
12cc syringe, 22 g needle
3cc syringe, 22 g needle
Lidocaine’EDTA
11 blade
slides
pietri dish
Red top tube
How do you perform a bone marrow biopsy?
Position Patient
Clip and Prep
Sterile gloves (12cc syringe and 3cc syringe)
22g needle to 12cc
2ml EDTA and lidocaine
EDTA flush through bone marrow needle
Lidocaine block
stab incision
place needle on bone
rotate back and forth with pressure
Seated move to confirm
Remove stylet and add 12G suction marrow
Stylet back
In dish and swirl (spicules = good) - omn slide
What things should be in a good bone marrow sample?
Megakarocytes and spicules
What would you perform a bone marrow biopsy?
Myelofibrosis
Same bone marrow aspirate site
What supplies do you need for a bone marrow biopsy?
Similar aspirate plus jamshidi needle
What is the difference in BMA and BMB?
BMB
-Needle advanced once stylet removed (cm), rock, remove from bone while twisting
Shepherd hook to remove it
What would a thoracocenteses be perfromed?
Collect pleural effusion for cytologic analysis
Relieve dyspnea caused by pleaural effusion or pneumothorax
What are contraindicaitons for thoracocenteses?
Coagulopathy
What are risks of throacoentesis?
Lung puncture or laceration
Hemorrhage
Pyothorax
What supplies are needed for throacocenetis?
Scrub
Sterile gloves
Butterfly needle
3 way stop cock
extension set
60cc syringe
How do you perform thoracocenteses ?
restrained lateral
find 7-9th intercostal space(near costochondral junction for fluid, dorsal for air)
Clip and prep
Sterile gloves
Butterfly with extension
Ultrasound to help pass
Bevel needle facing cranial
needle caudal bevel to center chest
Aspirate fluid or air
What are indications for abdominocentesis?
Collect abdonimal effusion for cytologic exam
Relieve clinical signs of dyspnea or discomfort
What are contraindications for abdominocentesis?
Coagulopathy
What are risks for abdominocentesis?
Perforation or laceration abdominal organs
Septic abdomen
What supplies are needed for thoracocentesis?
Scrub, sterile gloves, butterfly
syringe
How do you perform abdominocentesis?
left lateral or stand
Clip and prep
sterile gloves
needle to syringe 1-2cm lateral and caudal umbilicus
Gentle suction
Redirect needle
What are indications for arthrocentesis?
Joint swelling or pain
Shift leg lameness
Fever unknown origin
What are contraindications arthrocentesis?
Coagulopathy
Pyoderma at site
What are risks of arthrocenteiss?
Septic arthritis
Hemarthrosis
Where are common sites of arthrtodesis?
Carpus (radiocarpal)
Stifle
Tarsus
What is the procedure for arthrocentiss?
Clip and prep, sterile gloves, hold limb in flex, extend
palpate
needle
drop synovial fluid
View on slide
smear
