L7- iPSC research

Question 1

what is cell reprogramming

Answer 1

process of reverting mature, specialised cells into induced pluripotent stem cells.

Question 2

Things need to be considered when dealing with ESCs clinically:

Answer 2

• process of differentiation
• possibility of tumour formation
• Immune rejection -> iPSCs can be the solution
• genomic instability
• Ethical problems -> iPSCs can be the solution

Question 3

what are iPSCs

Answer 3

pluripotent stem cell derived from adult somatic cells that have been genetically reprogrammed to an embryonic stem (ES) cell-like state

Question 4

benefits of using iPSCs

Answer 4

• have the potential to replace the damaged tissues
• can self-replicate for long time
• can assist in how SCs develop into tissues > allowing for the search of the cures
• no immune rejection

Question 5

what TFs are used to maintain the embyronic stem cell state in iPSCs

Answer 5

OCT4, Sox2, klf4, C-myc,

Question 6

function of Klf4

Answer 6

contains zinc fingers

- inhibits the cell porliferation by controlling the expression of cellcycle

Question 7

function of C-myc

Answer 7

a protooncogene

- regulates, cell cycle, self renewal, pluripotentcy