L6- SCNT

Question 1

definition of cloning

Answer 1

• technique to create genetically identical organism

Question 2

types of cloning

Answer 2

embyro
reproductive
therapeutic

Question 3

what is embyro cloning

Answer 3

-natural process where the fertilised oocyte split up, producing twins

Question 4

what is embyro splitting

Answer 4

artifical splitting of the embyro > making few clones

Question 5

what is reproductive cloning

Answer 5

genetic material from a somatic cell put into another enucleated oocyte (SCNT)
- creating clones of the adult / parent organism

Question 6

what is therapeutic cloning

Answer 6

• when SC are used in vitro to grow human tissues

Question 7

how did cloning of dolly happen as in the events

Answer 7

1. enucleate the eggs from scottish blackface ewes
2. donor cells added to the oocyte in the culture
3. electricity given to make them fuse and start fertilsiation.
4. implanted in a surrogate

Question 8

problems for cloned embyros

Answer 8

can get developmental defects with enalrged placenta

- die during gestation.

Question 9

how are the SC cultured in therapeutic cloning

Answer 9

1. SCNT embyro or from a fertilised embyro
2. the embyro develop SC
3. the SC are removed
4. SC grown into specific tissues

Question 10

advantages of animal cloning

Answer 10

• genetically identical in their good features like high yield of milk
• preserving endangered animals
• imporve the quality of the herds

Question 11

disad of animal cloning

Answer 11

• have susceptibility to disease

- sucsess rate is quite low