L22: Pigments Flashcards

1
Q

Discuss light penetration vs depth

A

longer wavelengths dont penetrate as deepy as shorter ones, they deeper they penetrate the stronger they are (short wavelengths are more powerful)

*cyanobacteria absorbs shorter wavelegnths, can also absorb low

*deep organisms like prochlorococcus have pigments that absorb short wavelengths

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2
Q

What are photosynthetic pigements

A

*pigments to absorb light over range of wavelengths of available light in their specific niche
*chlorophylls, bacteriochlorophylls, carotenoids, phycobiliproteins

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3
Q

What is the black thing in soil column, what can it the colours depends on

A

black is sulphate input

changes with tide, bringing nutrients and other things can affect stratification. different nutrients, different niche, different colour

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4
Q

What is the peach colour

A

peach layer below purple are both purple sulfur of different niches

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5
Q

draw the wavelengths of cyano, PB and GB

A

draw, slide 76

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6
Q

In words, describe distribution of phototrophs by depth

A

UPPER ZONE
Surface: cyanobacteria, Chl a harvests shortest wavelengths of light
=>have gas vacules for buyancu, higher energy, PS2 can oxidise H2)

LOWER ZONE
Middle: PB, BchlA+B harvest longest wavelengths of light, long wavelengths cant penetrate any deeper => this is the limit for those organisms

deepest: GB, Bchl c, d, e, g harvest midrange wavelengths of light, the wavelengths penetrate deeper than those of PB meaning GB can be deeper than PS, in H2S zone away from O2

overall lower zone
=>PS absorb lower energy WLs so PS cannot oxidise H20, microbes can support
=>purple sulfur microbes support hetetrophs in that zone
=>settling organic matter supplies non phototrophs in deeper zones

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7
Q

What are key environmental factors and how do they impact microbial physiology
trOphs

A

lower tolernace limit /// upper tolerance limit

temperature: -12C /// 110C

redox potenital: -450 /// 850

ph 0 /// 13

hydrostatic pressure 0 /// 1400

salinity 0 /// saturated

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8
Q

methanogenic bacteria

A

most e-negative and aneorbic

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9
Q

What are crypoendothilic bacteria

A

hidden inside rocks
light can penetrate so it allows phototrophs / other microbes to grow, rocks can also retain small water (serves as protection)
lots of mineral ozidation

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10
Q

Associate environmental ranges with bacteria type

A

psychrophileS: -15 to below 10

mesophiles: 4 to 50

thermophiles: 40 to 100

a meso optimal would be minima for psychro

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11
Q

How do extreme psychrophiles survive

A

metabolic activity in brine veins between ice crystal
brines have salts, concentrated organic compounds, can be colder than -15

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12
Q

discuss extremes of pH

A

usually between 4 - 9 but can go to extremes
acidophiles, neutrophiles and alkaliphiles

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13
Q

Is vinegar made aerobically or anaerobically

A

Vinger = acetic acid from aerobic oxidation of ethanol by Acetobacter which decreases pH to 3, can also be produced via aerobic respiration via fermentation

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14
Q

high pressure high temp zones

A

thermal vents

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15
Q

What happens with acid mine drainage, what are ecological effects

A

coal, ores, tailings have reduced sulfur compounds like iron pyrate FeS2

=>they become spontaneously oxidised by O2 and by microbes acid mine drainage reactions, this drops pH to 4.5

=>iron bacteria active until too acidic, below 3.5 acidophiles take over

=>becomes oxidised to sulfuric acid H2SO4 and toxic metallic compounds known as Yellow boy

=> yellow boy becomes exposed to air

Ecological effects: H2O, iron compounds, ferric hydroxide precipitates run off into streams, kills all life, water undrinkable

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16
Q

What are the relevant oxidation states of iron

A

Fe2+ ferrous iron (Reduced, e- acceptor)

Fe3+ ferric iron (oxidised, e donor)

17
Q

Which microbes present at which steps of AMD

A

around 4.5: iron bactera gallionella which are present in iron braids, it forms a tubrcle aneorobic environment. this conditions support SRBs that remove H from iron and reduce sulphate to hydrogen sulifde

around 3.5: acidophiles, acidobaccilus which oxides FeS2 and Fe for energy, they reduce O2 (e- acceptor)
producing toxic waste product FeSO4 and Fe(OH)3

18
Q

AMD reaction equation

A

FeS2 + O2 + H2_ => Fe(OH) + H2SO4

19
Q
A