L16

Question 1

where was myc initially discovered

Answer 1

myelocytomatosis

Question 2

myelocytomatosis promoter

Answer 2

weak promoter relative to human

Question 3

myelocytomatosis LTR

Answer 3

flanking qtr allows it to be integrated into cell genome

Question 4

why is v-myc powerful

Answer 4

very robust transformation

Question 5

does v-myc expressed in chicken cells cause tumor

Answer 5

yes

Question 6

ALV

Answer 6

virus that inserts itself right in front of Mac promoter - so it’s a weak promoter but it is constantly on

this is a retroviral promoter

Question 7

common translocation for c-myc

Answer 7

Ig heavy chain locus undergoes translocation with Myc - the enhancer region

therefore IgG enhancer is in front of Mac, and induces gene expression

Question 8

where is Myc IgG enhancer translocation often found

Answer 8

Burrito’s lymphoma, but not other cancers

Question 9

c-myc knockout mice result

Answer 9

embryonic lethal

Question 10

n-myc c-myc rescue

Answer 10

if you make c-myc knockout cells, they have decreased proliferation and things. almost all of that activity can be rescued by n-myc

Question 11

neoblastoma n-myc duplication

Answer 11

In neoblastoma, n-myc is often duplicated many many times (gene amplification)

Question 12

Myc deregulation through MapK

Answer 12

mutation allows ERK to phosphorylate Myc -> then Myc is more active