Jordans Notes 4

Question 1

What are the two important principles of all DNA extraction?

Answer 1

1. Lysis
2. Purification

Question 2

How variable are SNP loci?

Answer 2

Usually only 2 possible alleles
- not very variable
- sometimes 3

Question 3

What base pairs create a triple hydrogen bond?

Answer 3

C to G

Question 4

How is tissue maceration of plants done?

Answer 4

Freezing with liquid nitrogen first
then a mortar and pestle

Question 5

What is the best method of extraction for undamaged DNA?

Answer 5

Silica magnetic beads

Question 6

What extraction method isn’t very good at removing inhibitors?

Answer 6

Chelex

Question 7

What is the side product when a nucleoside triphosphate is bound to a DNA strand?

Answer 7

A diphosphate group

Question 8

What is a determining factor of annealing temperature?

Answer 8

G+C content (triple hydrogen bonds)

Question 9

How many cycles are usually done with a PCR?

Answer 9

30 - 40

Question 10

Which end of the electrophoresis tank is the DNA placed in?

Answer 10

The negative end

because DNA is also negatively charged so is repelled towards the positive end