Jordans Notes 3

Question 1

What are the two steps to cause lysis?

Answer 1

1. Tissue maceration (mechanical breakdown)
2. Heating in buffer (increases cell breakdown) -> detergent, proteinase K, chaotropic salts

Question 2

What are the beads that DNA wrap around?

Answer 2

Histones

Question 3

If you precipitate a solution, what are you doing?

Answer 3

Causing a solid substance to separate from a solution

Question 4

A group with an ancestor but not all descendants?

Answer 4

Paraphyletic
- Reptilia without including birds
- - lizards without snakes

Question 5

What is the liquid left over from precipitation/centrifugation?

Answer 5

Supernatant

Question 6

Which DNA extraction has no separate lysis and purification step?

Answer 6

Chelex extraction

Question 7

What temperature is ok for short term DNA storage?

Answer 7

4 degrees C refrigeration

Question 8

How many phosphate groups does a nucleotide have?

Answer 8

1

Question 9

If you don’t know what species may be present in a sample, what kind of PCR primers should be used?

Answer 9

Universal primers

Question 10

Left over unused primers in PCR product

Answer 10

Primer dimers
not a huge issue