Joints, Cartilage, Synovium Flashcards

1
Q

Joints

(3 main types)

A

Provide articulation between bony elements

Main types:

•Synovial joints (synovial fluid-filled cavity)- can be a lot of fluid or not much

-Elbow, shoulder, stifle, hip, etc.

•Fibrous joints (~ligamentous tissue in gap)

  • Sutures of skull
  • Syndesmoses between equine metacarpal bones
  • Gomphosis holds tooth to bone of socket

•Cartilagenous joints (cartilage in gap)

  • Synchondroses at growth plate between epiphysis and diaphysis
  • Symphyses at midline (mandible, pelvis) and between vertebral bodies: cartilage on bone and fibro(cartilageno)us in middle

*what is different is what is in the gap between the two bones

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2
Q

Suture Joints

A

Sites of skull growth

-Fiberous tissue between the gap!

  • Membranes grow
  • Bone grows into membrane at suture

Allow some flexibility during parturition (giving birth)

In most species (not mice!) tend to close in adults: becomes bone

That is why a mouse can get through a hole the size of a standard biro pen

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3
Q

Sydesmosis and Gomphosis

(Fibrous Joints)

A
  • Bands of short ligaments holding structures tightly together
  • A syndesmosis is a slightly movable fibrous joint in which bones such as the tibia and fibula are joined together by connective tissue.–> (e.g. between horse metacarpals…2 and 4 are held tightly on the sides of the 3rd main digit)–>The syndesmosis between equine metacarpals can ossify (turn into bone/bony tissue) - this may be inflammatory and painful.
  • A gomphosis, also known as a dentoalveolar syndesmosis, is a joint that binds the teeth to bony teeth sockets in the maxillary bone and mandible. Forming articulation between tooth and mandible–> collectively gomphosis. (periodontal ligament)
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4
Q

Synchondrosis

(Cartilaginous Joint)

A
  • an almost immovable joint between bones bound by a layer of cartilage
  • Cartilagenous joint between epiphysis and diaphysis (growth plate)
  • Epiphyseal Plate (Growth plate) is a synchondrosis
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5
Q

Epiphyseal Plate

(growth plate)

A
  • The epiphyseal plate (or epiphysial plate, physis, or growthplate) is a hyaline cartilage plate in the metaphysis at each end of a long bone.
  • This ‘growth plate scar’ can persist for some time in young adult animals, after growth in length has stopped. Its main relevance to you is that it can look like a fracture radiographically, and fractures (Salter Harris types) can occur in this weak spot.
  • When a child’s bones are done growing (called reaching skeletal maturity), the growth plates ossify (harden) and the epiphysis fuses with the metaphysis, forming one complete bone
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6
Q

Symphysis

(cartilaginous Joint)

A
  • Mandibular: hold mandible together in the middle
  • In some animals mobile for mastication (chewing where food is crushed by teeth)
  • In most relatively stiff joint- rabbits it is quite mobile
  • Common traumatic injury in cats is mandibular symphysis fracture –> when cats fall and hit head/hit chin on ground, usually heals fine
  • Pelvic
  • Another common cat fracture site (with the sacroiliac joint, but that’s an unusual synovial joint)
  • Becomes softer/separated prior to parturition under influence of relaxin (from corpus luteum or placenta, depending on species–> cows and female humans)- sometimes even stays loose after
  • What about birds?

–>Birds & reptiles don’t have a complete pelvis with ventral midline fusion (as mammals do), which aids in the laying of eggs.

  • Symphysis ossification may occur with age and especially so in males.
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7
Q

Synovial Joint

(range of motion)

6 different shapes

A

Range of motion

•Defined in large part by shape of articular surfaces

A.Plane

B.Hinge (metacarpal/phalangeal joint- like surfaces of cylinder and opposite to cylinder –> can only rotate in that one plane, stops joint from going medial laterally)

C.Pivot

D.Condylar (e.g. knee, similar in that it stays in one plane or it may rupture. With a fracture of these joints, can note that movement becomes abnormal)

E.Ellipsoidal

F.Saddle

G.Spheroidal (e.g. hip is ball and socket joint–> needs to move in many different directions so needs spherical interface)

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8
Q

Motion of Synovial Joints

*IMPORTANT*

A

Main Kinds of Movement

  • Flexion / extension / hyper(over)extension: opening and closing angle of a joint

–> when a horse is standing, it’s in a hyperextended position, especially when galloping. Fully extended would be just a straight limb (180 degrees) *Remember which side to measure angle on- point of flexion*

  • Adduction / abduction
  • Circumduction
  • Rotation:

—>Pronation & supination

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9
Q

Flexion of joint

A

closing the angle of a joint

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10
Q

Extension of a Joint

A

Opening the angle of a joint

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11
Q

Adduction/Abduction

A

Adduction: moving towards midline/sggital plane

Abduction: moving away

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12
Q
A
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13
Q

Effective Mechanical Advantage of Joints

A

-turn small musclular motions into large limb motions

Effective mechanical advantage

  • Leverage provided by joint configuration
  • Ground reaction force (relates to mass of animal) vs. muscular force (muscle force must be relatively greater than the GRF to balance out the weight of animal bc the lever arm) - advantage is you get MUCH more movement
  • GRF: weight of animal causes a moment around the joint (being driven by external force(s) to cause the rotation
    example: calf muscle provides force (F)

Lever arms: (see-saw example)

  • r = distance from muscle insertion to fulcrum (the point against which a lever is placed on which it turns or is supported)
  • R = distance from contact point to fulcrum

fulcrum acts on opposite side of two forces that are working opposite

  • can have muscle closer to the fulcrum than where the work is happening in some joints
  • muscles can actually be distant from joints and lever arm. work can be done in muslce and change in shape of that muscle is minimal but causes a great movement of the limb
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14
Q

EMA

(Effective Mechanical Motion)

A

EMA= r/R

r= distance from muscle insertion to fulcrum

R= distance from contact point to fulcrum

  • Crouched animals have lower EMA (big R is bigger) and therefore less GRF-resisting moment. But, they tend to be more agile.
  • Large animals tend to adopt a less crouched or upright posture to increase EMA (by reducing R),
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15
Q

Synovial Fluid

A

The lubricant of synovial joints

  • Water
  • Hyaluronic acid
  • Glycoproteins
  • Surface active phospholipids
  • A few mononuclear leukocytes (WBCs)
  • glucose and electrolytes occur in concentrations similar to those in plasma (equilibrium with plasma)
  • Produced mainly by type B synoviocytes
  • Cartilage’s extracellular fluid: Transport of metabolites, waste, signaling molecules

-articular cartilage with metabolic transport

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16
Q

Synovia

A
  • 100 µm thick:
  • Superficial 2-cell layer
  • Subjacent loose fibrous/fatty tissue
  • Type A cells (macrophage-like)
  • Type B cells (fibroblast-like)
  • No basement membrane
  • Phagocytosis (type A cells)
  • Produce synovial fluid (type B cells)
  • Filter plasma (acts as plasma filtrate)

< 10kDa molecules get into synovial fluid from blood

17
Q

Synovial Sheaths

A
  • Can find synovia in other places such as tendons and ligaments
  • Provide lubrication to tendons as they pass over joints
  • After trauma, poor healing can lead to adhesion and loss of function

-synovia do not heal very well and tend to form a scar

18
Q

Joint Cavities

A
  • In complex joints like the equine carpus, there can be multiple synovial cavities in a single functional ‘joint’
  • Which might or might not communicate(shared synovial cavity): if one gets an infection the others may not
  • Depending on species, individual, disease…
19
Q

Articular Cartilage

A

_-Chondrocytes (_a cell which has secreted the matrix of cartilage and become embedded in it)

  • Sparse
  • In lacunae

-Extracellular matrix

  • Water (~70%ww)
  • Cartilage is predominantly Type II collagen (~60%dw)
  • Type I col. in fibrocartilage
  • Aggrecan

-Avascular

•But sometimes canals

-Perichondrium found on some cartilage but not articular cartilage

-Mineralises from deep surface

-Replaced by bone

  • Only partly
  • Leaving unmineralised HAC layer

NOTE:

  • thin layer of mineralized cartilage, softer cartilage near articular surface. Constant progression of bone invading into articular cartilage mediated by calcified cartilage layer
  • Take note of the collagen fibre orientation and consider the mechanical function in the radial, oblique and tangential zones.
20
Q

Cartilage Functions

A

*Resists compression

*Provides tough, low-friction bearing surface

-Resists shear

*Provides support

*Provides flexibility

*Functions supported by composition and arrangement of tissue

-Differently loaded parts of a joint have different composition

21
Q

Job of Hyaluronic Acid

A
  • acts as a linker protein
  • attaches proteoglycans to it which are entrapped into collagen network
  • the proteoglycan branches are hydrophilic (attract water), but repel themselves. Expand and attract water
  • very large and don’t move much
22
Q

Cartilage Lubrication

A
  • like two sponges being squeezed together, fluid layer between them when compressed, but cartilage also absorbs fluid
  • Different models:
  • Weeping
  • Fluid boundary layer
  • Breaks down if:
  • Synovial fluid loses it’s viscoelastic properties (e.g. inflamed synovium allowing more exchange with plasma)
  • Surface of cartilage is roughened e.g. worn and fibrillated

–> can lead to osteoarthritis

23
Q

Proteoglycans

A
  • Very large molecular weight molecules
  • Aggrecan abundant PG in cartilage
  • Negatively charged: repel self, attract water
  • High compressive modulus
24
Q

Synovial Joint Development

A
  • Cellular condensations differentiate into cartilage anlagen (will later become bones)
  • One anlage per future bone (anlage: site of future growth and development)
  • Interzone *
  • Gap between adjacent anlagen filled with undifferentiated mesenchyme
  • Cavitation
  • Interzone cell populations split and form articular cartilage (red; as distinct from growth cartilage of the anlagen)
  • 2 different cartilages with 2 different fates: one will become bone, one becomes articular cartilage
  • BMP Receptor Signaling Is Required for Postnatal Maintenance of Articular Cartilage
25
Q

Other Cartilages

A
  • Nasal: keeps nostral open
  • Ear
  • Trachea: hold trachea open–> has perichondrium that sits around the cartilage
  • Entheses

-Will deal with this in ligament and tendon

  • Ribs (costal cartilages): ventral aspect of ribs
  • Growth plate
  • Hyaline cartilage surrounded by perichondrium*

-Except hyaline articular cartilage

26
Q

Elastic Cartilage

A
  • Offers additional recoil (hence elastic)
  • Greater toughness in bending, less plastic
  • Found in larynx, ear, Eustachian tube, epiglottis
  • Elastic cartilage of epiglottis contains large quantities of elastin and offers additional flexibility
  • Large quantities of elastin
  • Surrounded by perichondrium
  • Often merges with higher modulus (stiffer) connective tissues for support
27
Q

Fibrocartilage

A
  • Mix between tendon, ligament (Type I collagen) and cartilage (Type II collagen)
  • Additional resistance to tension
  • Higher tensile modulus, stiffer in tension
  • Found in intervertebral disc, tendon, ligament attachment sites, joint menisci
  • find in places where there is not just compression and shear, but where there is tension as well
  • e.g. femorotibial, temporomandibular joints
  • type-I collagen in bundles, less proteoglycan than hyaline cartilage
  • No perichondrium
  • Fibrochondrocytes between ECM bundles
28
Q

Intevertabal Disk

A
  • Nucleus pulposus surrounded by fibrocartilage anulus fibrosus
  • Common site of injury
  • tends to calcify in some breeds and species
  • Dachshund particularly susceptible due to abnormal cartilage (NP calcifies) and short leg/long back locomotor style