Isoalted Tissue

Question 0

What parameters can be measured?

Answer 0

Changes in force, flow or pressure
Release of substances into the perfusate
Oxygen consumption/ carbon dioxide production
Electrophysiological changes

Question 1

Why used isolated tissue?

Answer 1

Convenient

Easily subjected to controlled changes

Question 2

What are constituents in organ bath?

Answer 2

Physiological buffer (water based)
Physiol salt solution: Ringers, Tyrodes,Krebs Haseleit
Aeration: 95% oxygen and 5% carbon dioxide

Question 3

How should tissue be collected?

Answer 3

Fast dissection
Minimal handling
Dissected/transported on ice

Question 4

What tissues deteriorate fast?

Answer 4

Liver

Question 5

What tissues are stored in chilled buffet at 4oC in the fridge?

Answer 5

Guinea pig ileum (aerate)
Smooth muscle strips & rings
Papillary muscle

Question 6

What are limitations of isolated tissue preps?

Answer 6

Damage in isolating
No exact match to internal conditions of body
Delivery of oxygen & nutrients by diffusion (hypoxic core)