Health And Safety Flashcards
What should be used to prevent chemicals off the skin?
Use gloves
Lab coat
Shoes not sandals
What can happen if health and safety procedures are not followed in the laboratory?
Absorption, inhalation and ingestion of chemicals and biological agents Cuts, needle sticks Chemical and thermal burns Fire Explosion
What should be used to prevent inhalation of chemicals?
Fume cupboards
Respirators
How can ingestion of chemicals and biological agents be done?
By not washing hands properly
Eating/drinking on the laboratory
Musing reagents in the lab for food use
Pipetting by mouth
How can cuts from forcing a glass tubing into the hole of a stopper be prevented?
By lubricating the rubber stopper and using gentle pressure with rotation on the glass part. Always oil rather than push on the glass when possible
How can needle stick injuries be prevented?
Not properly re-sheath or recap a needle
What type of chemicals are corrosive to the skin and eyes?
Many chemicals such as mineral acids and alkalis
How are thermal burns done by?
Hot plates
What would cause an explosion?
Heating or conducting a reaction in a Closed system
What can prevent a explosion?
System which is vented
What are risk assessments involve?
Identifying the risk
Evaluating the risk
Minimising the risk
What does COSHH involve?
Reagents (substances) used
What does COSHH stand for?
Control of Substances Hazardous to Health
What substances does COSHH include?
Chemicals Products containing chemicals Fumes Dusts Vapours Mists Nanotechnology Gases and asphyxiating gases and Biological agents
What does COSHH not cover?
Lead
Asbestos
Radioactive substances
What does bio safety level 1 involve?
Basic protection
Most laboratories
Agents that known to cause disease to humans
What does biosafety 2 involve?
Moderate risk agents known cause human disease by ingestion, percutaneous, or mucus membrane exposure
What does biosafety 3 involve?
Indigenous or exotic agents with a know potential for aerosol transmission
Agents that may cause serious/ potentially lethal infections
What does biosafety 4 involve?
Exotic agents with high individual risk of life threatening disease by infectious aerosols for which non treatment is available
Lists some biohazards
Animal products/tissues Humans products/tissue Viruses Components of the cell culture media Cell products Transformed cells/cells contains recombinant DNA (modified or deactivated viruses and hybrid proteins)
What are hypochlorites?
General purpose
Active against viruses
2500 ppm
What does alcohols do as disinfection agents?
70% effective concentration
Dehydrates and fixes
Effective against bacteria
Ethanol is effective against most viruses but not non-enveloped viruses
How can aldehydes be used as disinfection?
Can cause extrem sensitisation
Use only on well-ventilated areas
How should biohazards be waste disposed?
Inactivate 2 hrs in hypochlorite solution (10,000 ppm)
Contaminated pipettes should be paved overnight in hypochlorite solution (2500 ppm)
Autoclave get waste
Incineration
