Health And Safety

Question 0

What should be used to prevent chemicals off the skin?

Answer 0

Use gloves
Lab coat
Shoes not sandals

Question 1

What can happen if health and safety procedures are not followed in the laboratory?

Answer 1

Absorption, inhalation and ingestion of chemicals and biological agents
Cuts, needle sticks
Chemical and thermal burns
Fire 
Explosion

Question 2

What should be used to prevent inhalation of chemicals?

Answer 2

Fume cupboards

Respirators

Question 3

How can ingestion of chemicals and biological agents be done?

Answer 3

By not washing hands properly
Eating/drinking on the laboratory
Musing reagents in the lab for food use
Pipetting by mouth

Question 4

How can cuts from forcing a glass tubing into the hole of a stopper be prevented?

Answer 4

By lubricating the rubber stopper and using gentle pressure with rotation on the glass part. Always oil rather than push on the glass when possible

Question 5

How can needle stick injuries be prevented?

Answer 5

Not properly re-sheath or recap a needle

Question 6

What type of chemicals are corrosive to the skin and eyes?

Answer 6

Many chemicals such as mineral acids and alkalis

Question 7

How are thermal burns done by?

Answer 7

Hot plates

Question 8

What would cause an explosion?

Answer 8

Heating or conducting a reaction in a Closed system

Question 9

What can prevent a explosion?

Answer 9

System which is vented

Question 10

What are risk assessments involve?

Answer 10

Identifying the risk
Evaluating the risk
Minimising the risk

Question 11

What does COSHH involve?

Answer 11

Reagents (substances) used

Question 12

What does COSHH stand for?

Answer 12

Control of Substances Hazardous to Health

Question 13

What substances does COSHH include?

Answer 13

Chemicals
Products containing chemicals
Fumes
Dusts
Vapours
Mists
Nanotechnology 
Gases and asphyxiating gases and
Biological agents

Question 14

What does COSHH not cover?

Answer 14

Lead
Asbestos
Radioactive substances

Question 15

What does bio safety level 1 involve?

Answer 15

Basic protection
Most laboratories
Agents that known to cause disease to humans

Question 16

What does biosafety 2 involve?

Answer 16

Moderate risk agents known cause human disease by ingestion, percutaneous, or mucus membrane exposure

Question 17

What does biosafety 3 involve?

Answer 17

Indigenous or exotic agents with a know potential for aerosol transmission
Agents that may cause serious/ potentially lethal infections

Question 18

What does biosafety 4 involve?

Answer 18

Exotic agents with high individual risk of life threatening disease by infectious aerosols for which non treatment is available

Question 19

Lists some biohazards

Answer 19

Animal products/tissues
Humans products/tissue
Viruses
Components of the cell culture media
Cell products
Transformed cells/cells contains recombinant DNA (modified or deactivated viruses and hybrid proteins)

Question 20

What are hypochlorites?

Answer 20

General purpose
Active against viruses
2500 ppm

Question 21

What does alcohols do as disinfection agents?

Answer 21

70% effective concentration
Dehydrates and fixes
Effective against bacteria
Ethanol is effective against most viruses but not non-enveloped viruses

Question 22

How can aldehydes be used as disinfection?

Answer 22

Can cause extrem sensitisation

Use only on well-ventilated areas

Question 23

How should biohazards be waste disposed?

Answer 23

Inactivate 2 hrs in hypochlorite solution (10,000 ppm)
Contaminated pipettes should be paved overnight in hypochlorite solution (2500 ppm)
Autoclave get waste
Incineration