Irradiation, Leukoreduction, Washing

Question 1

Irradiated Products:

• Amount to midplane
• Minimum dose to any portion

Answer 1

• 25 Gy (2500 cGy) - Midplane

- 15 Gy (1500 cGy) - Any portion

Question 2

How does irradiation affect RBC storage/expiration?

Answer 2

28 days or the original outdate

-Whichever is sooner

Question 3

What is the indication for product Irradiation?

Answer 3

Prevention of TA-GVHD

Question 4

What is the usual method of leukoreduction?

Answer 4

Filtration

Question 5

To be labeled as leukoreduced, 95% of tested units must contain:

• RBCs/Apheresis platelets
• Whole Blood derived platelets

Answer 5

RBCs/Apheresis Platelets:
-<5x10^6 WBCs

Whole Blood Platelets:
-<8.3x10^5

Question 6

The leukoreduced Red Cell unit must retain ___% of the original Red Cells (whole blood derived) or ___ g of hemoglobin (Pheresis units).

Answer 6

> 85%

51 g

Question 7

Leukoreduction Indications. (4)

Answer 7

Prevention of:

• HLA alloimmunization
• Febrile, nonhemolytic, TxRxns
• CMV transmission
• Transfusion Related Immune Modulation (TRIM)

Question 8

T/F: Leukoreduction is NOT effecting in preventing TA-GVHD.

Answer 8

True

Question 9

What does Washing products remove?

Answer 9

Plasma

-Resuspended in normal saline

Question 10

What is the primary indication of Washed products? (2)

Answer 10

-Prevention of recurrent or Severe Allergic Reactions
(IgA defecient recipients)
-Transfusing neonates with cellular products bearing incompatible plasma

Question 11

What is the shelf-life of washed RBCs?

-Platelets

Answer 11

• 24 hours (washed RBCs)

- 4 hours (washed platelets)