Investigating Enzymes Flashcards
1
Q
When investigating the affect of temperature on enzyme activity what could you measure?
A
How fast a product appears How fast a substrate disappears
2
Q
How would you measure how fast a product appears?
A
- The enzyme catalase (potato) catalyses the breakdown of hydrogen peroxide into water and oxygen.
- Collect and measure how much oxygen is produced in a set time.
- Use a pipette to add a set amount of hydrogen peroxide to a boiling tube. Put the tube in a water bath at 10*c.
- Add 1cm3 catalase (potato) to hydrogen peroxide and quickly attach a bung.
- Record how much oxygen is produced in the first minute. Repeat 3 times and calculate the mean.
- Repeat at 20*c, 30*c & 40*c
- Control any variables e.g. pH, the potato used, size of potato pieces etc. To make it a fair test.
3
Q
How would you measure how fast a substrate disappears?
A
- The enzyme amylase catalyses the breakdown of starch to maltose.
- It’s easy to detect starch using iodine solution- if starch is present iodine changes from brown to blue/black.
- Put a drop of iodine in each spot. Every 10 seconds drop a sample of the mixture into a well on the spotting tile using a pipette. When the iodine solution remains brown record the total time taken.
- Repeat with the water bath at different temperatures to see how it affects the time taken for the starch to be broken down.
- Remember to control all of the variables each time.
4
Q
What else can you investigate regarding enzymes?
A
pH
