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Histology 1 > Introduction to Histology > Flashcards

Introduction to Histology Flashcards

1
Q

What is histology?

A

The microscopic study of normal cells and tissue

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2
Q

What is pathology or histopathology?

A

Study of diseased cells and tissue

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3
Q

What microscopy is used to see the ultrastructure of tissues and cells?

A

Electron microscopy

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4
Q

What microscopy is most commonly used for histopathology?

A

Light microscopy

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5
Q

What biopsy is used for skin/oral?

A

Punch/incision

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6
Q

What biopsy is used for invisible lumps/organs?

A

Needle with MRI/X-Ray

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7
Q

What biopsy is used for colon/bladder/lung?

A

Endoscopic

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8
Q

What tissue does Masson trichrome stain?

A

Connective tissue

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9
Q

What does H&E stain?

A

Haematoxylin stains acidic structures such as nucleic acids blue/purple and eosin stains basic structures such as cytoplasmic proteins red/pink

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10
Q

What does Masson trichrome stain blue/purple?

A

Basophilic structures such as nucleic acids

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11
Q

What does Masson trichrome stain red?

A

Muscle, red blood cells, keratin, cytoplasm

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12
Q

What does Masson trichrome stain green/blue?

A

collagen

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13
Q

What does reticulin stain, stain?

A

Reticular fibres found in connective tissues black/blue

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14
Q

What microscopy is most compatible with staining?

A

Light

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15
Q

What are the stages of microscopic examination?

A

Specimen collection, fixation, dehydration, embedding, sectioning and staining

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16
Q

What is paraffin not compatible with?

A

alcohol/water

16
Q

What is staining not compatible with and what is used to remove it?

A

xylene is used to remove paraffin
alcohol is used to remove xylene
water is used to remove alcohol

17
Q

What does PAS stain?

A

complex carbohydrates, mucus from goblet cells, brush borders, basement membranes all magenta

18
Q

What is the most common type of stain?

A

H&E

19
Q

How does immunohistochemistry work?

A

Antibody conjugated to an indicator (enzymatic or fluorescent) specific to an antigen that is is trying to detect

20
Q

What stain identifies functionality?

A

Immunohistochemistry

21
Q

What does alcian blue stain?

A

Stains mucin and cartilage blue.

22
Q

What does vie gieson stain?

A

collagen red

nuclei, RBCs, cytoplasm yellow

23
Q

What device is used for sectioning and how thick are the sections?

A

7um thick sections cut using microtome, mounted on to glass slide

24
Q

What is fixation and what chemicals are used?

A

Preserves the structural arrangement, terminates all biochemical reactions and prevents tissue decomposition.

Uses formaldehyde and glutaraldehyde

Histology 1 (8 decks)

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