Introduction/overview Flashcards
What are the domains of a transcription factor?
DNA binding do, inactivation domain, activation domain, interaction domain
What is asymmetric division of a stem cell?
One daughter cell differentiates while the other becomes pluripotent
Where does most of the RNA in the early oocyte come from?
Egg
What can TFs do?
Bring pieces of DNA close together, bend the DNA, bind to the DNA
What happens as a result of Sox2 and Oct4 binding?
activation of pluripotency genes
What does a consensus binding site image represent?
The frequency of a nucleotide appearing at each position in a DNA sequence
General role of TFs?
Recruiting the RNA polymerase
How are events at the cell membrane linked to events at the nucleus?
A receptor on CSM is bound to, leads to a kinase cascade, TF may be phosphorylated, TF does its stuff in the nucleus
How might a DNA sequence with no mutation in the coding region not be able to produce a protein
Mutation in TF binding site
Three conformations of chromatin?
closed, permissive, open
Why can TFs bind to DNA in open chromatin conformation?
Enough space between for nucleosomes for them to fit
Difference between permissive chromatin and open chromatin?
Not all TFs have bound to the chromatin, and/or polymerase isnt present
How many histones make up a nucleosome?
8
