Intro to Microscopy Flashcards
visual enlargement of an object
magnification
refers to the extent to which the detail of a magnified object is maintained/ clarity of magnified object
resolution
the object will appear dark against a bright background through a condenser that allows light to pass through in parallel waves
bright field microscope
the object will appear bright with a dark background because of a special sub-stage condenser that causes light waves to cross rather than pass through the specimen
dark field microscope
has a special exciter filter which allows short wavelength blue light to pass and cross the specimen
fluorescent microscope
allows the detection of objects exhibits birefringence or refraction of light into two slightly different positions
polarizing microscope
slows down the speed of light by half a wavelength, retardation of light rays diffused by the object resulting in the halo effect allowing observation of unstained preparations
phase contrast microscopes
three dimensional and layer by layer image of the specimen that appears bright against dark background
interference contrast microscope
utilizes electron beams instead of light rays and magnetic fields instead of lenses allowing user to view extremely small particles such as viruses
electron microscope
type of electron microscope which allows the user to view the external microstructures of the particulate matter
scanning electron microscope
type of electron microscope which allows the user to view the internal microstructures of the particulate matter
transmission electron microscope
generates the initial magnification of the image
objectives
scanning objective (red) power
4x
LPO (yellow) power
10x
HPO (blue) power
40x
Oil Immersion power
100x
elevates or lowers the stage to roughly focus the specimen
coarse adjustment knob
elevates or lowers the stage in smaller increments or premeasured distances to refine focus after shifting to higher objectives
fine adjustment knob
holds the specimen in place
stage and stage clip
directs light in parallel waves through the specimen
condenser
moves the specimen vertically
specimen holder Y-axis feed knob
moves the specimen horizontally
specimen holder X-axis feed knob
compensates for the differences in eyesight between your eyes
diopter adjustment ring
allows the user to shift objectives
revolving nosepieces
controls the mechanism for preventing collision between the specimen and objective
pre-focusing knob
