Intro to Histology

Question 1

Hematoxylin Stain

• What is it’s charge in solution?
• What does it bind to?
• What does it stain?
• What is the terminology for this stain?

Answer 1

• +
• Negatively charged materials
• Nucleus, phosphates, sulfates

Question 2

Eosin Stain

• What is the charge of this stain in solution?
• What does it bind to?
• What is the name for this type of stain?

Answer 2

• -
• Positvely charged materials, i.e. proteins
• Acidophilic

Question 3

Selective Stains

• Trichrome
• Silver
• Periodic Acid Schiff (PAS)

Answer 3

• Collagen
• PROTEINS that are highly glycosylated
• Carbohydrates and glycosaminoglycans (glycocalyx)