Chromatin Structure Flashcards
What is a super coil?
The coiling of a coil. DNA is coiled in the form of a double helix around an axis. Further coiling of that axis produces a supercoil.
With regards to primary, secondary, tertiary and quaternary structure, what is a supercoil?
Intrinsic to DNA tertiary structure
What causes supercoiling to occur?
Results when DNA is subject to some form of structural strain. In most cases, this is due to underwinding of the DNA, which creates less stable DNA and puts thermodynamic strain on the DNA leading to supercoiling. Example: When DNA is being unwound by helicases, regions become underwound and create stress on other regions which become overwound in response.
What is the linking number?
The number of times a strand of DNA winds in the right handed direction around the helix axis
When is the linking # positive? Negative?
If interwound strands are wound as Right handed helix = positive If interwound strands are wound as left handed helix = negative
What is the equation for linking number?
base pairs / # bases per turn In DNA the denominator is always 10.5
What is chromatin
Amorphous form of DNA that consists of both proteins and DNA in approximately equal portions by mass
What does positive supercoiling mean? Negative?
+ = overwound - = underwound
What is a topoisomerase?
An enzyme that increases or decreases the extent of DNA underwinding. They change the linking number.
What is the relationship between linking number and supercoiling?
The higher the linking number, the more overwound the DNA is, the greater the degree of supercoiling
There are 2 types of topoisomerases. a) How do they effect linking number? b) By what means do they effect linking number? c) Do they use energy to function?
Type 1 - changes linking number by 1 by breaking 1 strand of DNA, passing the free end through the other strand and then rejoining the cut ends. No ATP needed. Type 2 - changes linking number by 2 units by breaking 2 strands of DNA, passing both through the helix and then re-sealing them. ATP is required.
Topo 1 is different in prokaryotes and eukaryotes. What are these differences?
Prokaryotes: only relax negative supercoil, only change link# in increments of +1 Eukaryotes: relax either positive or negative, can change in increments of -1 or +1
Topo 2 is different in prokaryotes and eukaryotes. What are these differences?
Prokaryotes: Introduce negative supercoil OR relax positive supercoil Eukaryotes: Only relaxes negative supercoils
When are topoisomerases needed? (6)
DNA Replication Transcription Recombination DNA Repair DNA Condensation Chromosomal Segregation
Describe the structure of chromatin.
Primary structure: 2nm DNA fiber wound around nucleosome to make 10nm fiber, “beads on string”
Secondary structure: 30nm fiber (nucleosomes fold in zig-zag fashion)
Tertiary structure: Nuclear scaffolding around non-histone scaffold proteins, (30nm fiber is thrown into irregular loops to form 300nm fiber, which then binds to protein scaffold that further coils DNA into rosettes forming 700nm fiber)
Quaternary structure: Condensed chromosome (700nm rosettes compress and stack on top of one another to form arms of chromosome)
Compare and contrast euchromatin and heterochromatin.
Describe how chromosomes localize within the nucleus.
Some chromosomes prefer to be located near the nuclear envelope, others near the proteins that support the nuclear envelope (called nuclear lamina), others dispersed through middle
Describe the forces that bind DNA to histones / nucleosomes.
The backbone of DNA is highly negatively charged due to phosphates in phosphodiester bonds, and histone proteins that make up the nucleosomes are highly positively charged, so the ionic interactions between these charges forms salt bridges that stabilize the binding of DNA to nucleosomes
Describe the structure of a nucleosome
A nucleosome is made up of 8 histones.
2 x H2A
2 x H2B
2 x H3
2 x H4
First, a tetramer (4) of 2xH3 and 2xH4 binds to the DNA, then 2 dimers of 1xH2A and 1xH2B bind
What is linker DNA? What is it’s relevance to histone H1?
Linker DNA is the DNA that is inbetween adjacent nucleosomes and histone H1 binds to that DNA (think of the spaces between the beads on the string)
What happens to the tertriary structure of the DNA when it binds to a nucleosome?
Binding to a nucleosome induces the formation of a negative supercoil (relaxes DNA, underwound)
Where do histones bind to DNA?
Areas with an abundance of A=T bases, which happens to be in minor groove usually, so histones bind to minor groove. This is necessary b/c this compresses the minor groove and makes it so DNA can wind tightly around the nucleosome at the minor groove areas leaving the major groove areas open and accessible for interaction with proteins.
What is histone H1?
Linker binding histone, it medaites interactions between adjacent histones / nucleosomes
What other proteins are important for the formation of the nucleosome from histones (think field trips)?
Chaperones
- Recruit histones to make nucleosome
- Prevent random non-specific association of histones with DNA
- Prevent formation of deleterious off path intermediates
