Intro to Histology

Question 1

4 basic steps of preparing a slide

Answer 1

1. Fixation
2. Embedding (plastic or paraffin)
3. Sectioning
4. Staining

Question 2

Hematoxylin

Answer 2

Blue, basic dye

Attracted to acidic cell components (RNA and DNA)

Question 3

RNA and DNA are ___ and stain ___

Answer 3

Basophilic

Stain blue

Question 4

Eosin

Answer 4

Pink, acidic dye

Attracted to basic cell components (cytoplasmic proteins)

Question 5

Cytoplasmic proteins are ____ and stain ____

Answer 5

Eosinophilic

Stain pink

Question 6

EM images are obtained with which heavy metal?

Answer 6

Osmium

Question 7

SER functions

Answer 7

Lipid production and metabolism
Production of steroid hormones
Detoxification of chemicals

Question 8

Functions of cristae in mitochondria

Answer 8

Increase the surface area of the inner membrane

Allows for increased ATP production

Question 9

3 complexes in a junctional complex

Answer 9

Tight junctions
Adherens junctions
Desmosomes

Question 10

2 types of anchoring junctions

Answer 10

Adherens junctions

Desmosomes (macula adherens)

Question 11

Tight junction

Answer 11

Occluding junctions/zonula occludens
Allow cells to form barriers
Located at the apical surface of cells
Prevent unregulated diffusion of water and other molecules

Question 12

Adherens junction

Answer 12

Link actin filaments within cells

Question 13

Demosomes

Answer 13

Link intermediate filaments within cells

Very strong

Question 14

Fuzzy plaque

Answer 14

Black (electron dense) material that appears along the cytoplasmic side of each cell in adherens junctions

Question 15

What is the space within the nuclear membrane called?

Answer 15

Perinuclear cistern