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Liz's Hematology Module 2 > Intro to Flow Cytometry > Flashcards

Intro to Flow Cytometry Flashcards

1
Q

CD3, CD5, CD7, CD4, CD8

A

T-cell markers

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2
Q

CD34, CD117, TdT

A

Immature markers

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3
Q

CD71, glycophorin A

A

Erythroid precursor marker

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4
Q

CD19, CD20, Kappa light chain, lambda light chain

A

B-cell marker

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5
Q

CD41, CD42, CD61

A

Megakaryocytes marker

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6
Q

CD13, CD14, CD15, CD33, CD34

A

Granulocytic/monocytic marker

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7
Q

Three most common applications of flow cytometry

A
  • Diagnosis and subclassification of leukemias and lymphomas
  • Detection of minimal residual disease before the overt relapse of acute leukemia
  • Provides additional information in morphologically challenging questions
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8
Q

Define hydrodynamic focusing

A

Process by which cells suspended in sheath fluid pass in front of a light source one at a time

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9
Q

Gating vs. acquisition of all events

A
  • Acquisition of all events is the comprehensive analysis of all cells
  • Gating is the process of drawing circles around the cell population that will be studied in greater detail
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10
Q

Which type of gating is most appropriate to use to investigate low incidence cell population?

A

Live/gating

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11
Q

What does forward scatter measure?

A

Cell size

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12
Q

What does side scatter measure?

A

Complexity of the cytoplasm (granularity and vacuoles)

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13
Q

Make sure you know

A
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14
Q

Make sure you know

A
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15
Q

Briefly explain how a flow cytometer works w/ respect to monoclonal Abs, fluorescent dyes, excitation by a laser, and detection

A

Fluorescently labeled monoclonal Abs to stain cell of interest

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16
Q

What type of cells are used for flow cytometry?

A
  • Granulocytes
  • Lymphs
  • Non-nucleated cells
  • Monocytes
  • Nucleated cells
  • Red cells
17
Q

Flow cytometry is capable of measuring…

A
  • Cytoplasmic Ags
  • Membrane-bound Ags
  • Nuclear Ag (DNA or RNA sequences)
  • Physical characteristics (size, morphological and cytoplasmic complexity)
18
Q

The term CD stands for…

A

Cluster Designation (or cluster of differentiation)

19
Q

The marker that is present on all hematopoietic cells and their progenitors except for megakaryocytes and erythroid cells is…

A

CD45

20
Q

What type of cells express CD5 but only dimly?

A
  • Plasma cells
  • Precursor B cells
  • Immature blasts
21
Q

What clinical situations is flow cytometry used for?

A
  • T cell subset of determination for diagnosis and follow-up in HIV positive patients
  • Detection of minimal residual disease before obvious relapse of acute leukemia
  • Diagnosis and subclassification of leukemias and lymphomas

Liz's Hematology Module 2 (13 decks)

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