Cytochemistry

Question 1

Staining constituents of cells to help determine lineage; used when cells are so immature that you cannot identify them from morphology alone

Answer 1

Cytochemistry

Question 2

Enzyme found in primary granules of neutrophils

• Positive in myeloid cells
• Negative in lymphoid cells

Answer 2

Myeloperoxidase stain (MPO)

Question 3

Stains lipids and is slightly less sensitive than MPO

• Positive in primary and secondary granules in neutrophils and monocytes
• Negative in lymphoid cells

Answer 3

Sudan Black Stain (SBB)

Question 4

Primarily used to identify early granulocytic cells where red indicates positive
- SPECIFIC ESTERASE

Answer 4

Naphthol-AS-D-Chloroacetate Esterase (NASDA)

Question 5

Primarily used to identify monocytic cells

- NONSPECIFIC ESTERASE

Answer 5

Alpha Naphthyl-Butyrate/Acetate Esterase (ANBE/ANAE)

Question 6

____ is positive in megakaryocytes while ____ is not

Answer 6

ANAE; ANBE

Question 7

In ANBE, red is positive in ____ cells and the ____ cells are negative

Answer 7

Monocytic/ myeloid

Question 8

Stains glycogen

Answer 8

Periodic Acid Schiff (PAS)

Question 9

Block positivity of PAS is present in what?

Answer 9

Blasts of ALL

Question 10

PAS is negative in normal RBC precursors, but positive in blasts for what?

Answer 10

Erythroid type of AML

Question 11

Immunoperoxidase reaction for Factor VIII is positive in what?

Answer 11

Megakaryoblastic leukemia

Question 12

MPO and SBB are positive for what cells?

Answer 12

Myeloid

Question 13

NASDA is specific and stains for what?

Answer 13

Granulocytes

Question 14

ANBE is non-specific and is positive in what?

Answer 14

Monocytic

Question 15

PAS is positive in what?

Answer 15

In ALL and leukemic or malignant RBCs