Interpretation, Evaluation and Statistics Flashcards
1
Q
How are DNA fragments sized?
A
They’re compared to an internal standard
2
Q
What is the most common form of additional peaks in an hlectropherogram?
A
Stutter products
3
Q
When a mixture is present what do we look for? (3)
A
More than 2 peaks at a locus, Higher than expected stutter levels and a severe imbalance in heterozygote peak sizes
4
Q
How many people should data be reviewed by?
A
At least 2
5
Q
What characterises spikes?
A
They are much narrower than normal and go through all dye channels
6
Q
What characterises dye blobs?
A
They are much broader than normal and only go through one channel
7
Q
Why are thresholds required?
A
So that we are confident with our analysis
