DNA Quantification, Separation and Detection Flashcards

1
Q

How much DNA does a commercial PCR kit require?

A

0-1ng

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2
Q

What distance is required to distinguish between alleles?

A

100-400bp

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3
Q

What size DNA fragments are run through aragose gel?

A

1500-2000bp

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4
Q

What size DNA fragments are run through polyacrylamide gel?

A

500-1000bp

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5
Q

What resolution do we require for DNA analysis?

A

1bp

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6
Q

What charge does DNA have?

A

Negative

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