DNA Quantification, Separation and Detection Flashcards
1
Q
How much DNA does a commercial PCR kit require?
A
0-1ng
2
Q
What distance is required to distinguish between alleles?
A
100-400bp
3
Q
What size DNA fragments are run through aragose gel?
A
1500-2000bp
4
Q
What size DNA fragments are run through polyacrylamide gel?
A
500-1000bp
5
Q
What resolution do we require for DNA analysis?
A
1bp
6
Q
What charge does DNA have?
A
Negative