Degraded DNA, Mixtrues, LCN and Parentage

Question 1

What is a consequence of more sensitive DNA tests?

Answer 1

Contamination

Question 2

What reduces as DNA becomes more degraded?

Answer 2

the amount of repeats of a full sequence of DNA

Question 3

What happens if more than 4 peaks are present at a loci?

Answer 3

It is a complex mixture of 2+ people

Question 4

What happens if we have too many PCR cycles?

Answer 4

Allele drop-in can occur

Question 5

What is allele drop-in?

Answer 5

Occurs when a peak shows on an hlectropherogram that isn’t an actual allele.

Question 6

What can degrade DNA? (5)

Answer 6

Time, environmental condition, humidity, biological activity and UV

Question 7

How will degraded DNA appear on an aragose gel?

Answer 7

It will smear

Question 8

Why were miniSTRs developed?

Answer 8

To detect alleles on degraded DNA

Question 9

How much DNA is needed to get a profile with miniSTR?

Answer 9

at least 10 cells (0.062ng)

Question 10

For heterozygotes, how much variation can occur in peaks?

Answer 10

Generally no more than 30%

Question 11

How large is stutter?

Answer 11

Generally no larger than 15%

Question 12

What is the equation for the minimum number of contributors?

Answer 12

number of alleles / 2