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HISTO > Instrumentation > Flashcards

Instrumentation Flashcards

1
Q

Used by histotechnologists for QC

A

microscope

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2
Q

Microtome:
Inventor:
Purpose: cutting serial sections of large block of paraffin; simplest

A

Rocking
Paldwell Trefall
“Na fall ka tungod sa rock”

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3
Q

Microtome:
Inventor:
Purpose: cutting embedded section; most common

A

Rotary
Minot
“routine”

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4
Q

Microtome:
Inventor:
Purpose: cutting celloidin; dangerous due to movable knife

A

Sliding (standard)
Adams
“na slide si adam”

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5
Q

Sliding microtome that is ideal for resin-embedded decalcified bone

A

base sledge (knife is stationary)

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6
Q

Microtome:
Inventor:
Purpose: cutting unembedded frozen sections

A

Freezing
Queckett
“quick-freeze”

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7
Q

Microtome:
Inventor:
Purpose: EM; embedded in plastic resins using diamond/glass knives

A

Ultrathin

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8
Q

Knife: Plane concave
Length:
Info:

A

25 mm
less concave = celloidin
more concave = paraffin

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9
Q

Knife:
Length: 120 mm
Info:

A

Biconcave
Rotary microtomes for paraffin

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10
Q

Knife:
Length:
Info: Both sides are straight for frozen & very tough paraffin embedded tissues

A

Plane wedge
100 mm

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11
Q

Knife:
Info: more commonly used nowadays; eliminates honing & stropping process

A

Disposable knives

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12
Q

Knife:
Info: suitable for cryostat

A

Magnetic

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13
Q

Knife:
Length: 0.5 - 1 um
Info:

A

Glass knives
semi-thin survey sections

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14
Q

Knife:
Length: 50-120 um
Info:

A

Diamond knives
ultra-thin sections for EM
brittle but very durable

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15
Q

Process of removing the “nicks”
Heel to toe direction (20-30 double strokes)

A

Honing

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16
Q

Type of hone:
Manual sharpening but gives best results

A

Belgium yellow

17
Q

Type of hone:
Gives more polishing effect

A

Arkansas

18
Q

Type of hones:
much coarser & for badly nicked knives

A

Fine carborundum

19
Q

Process of removing “burrs”, dine to polish & sharpen
Toe to Heel direction (40-120 double strokes)

A

Stropping (horse leather)

20
Q

Used to clean microtome

A

soft brush & xylol

21
Q

Bevel angle

A

27-32° angle

22
Q

Causes maximum penetration of tissues & minimize distortion

A

Perfect & optimum angle (15°)

23
Q

Angle is around 0-15° which prevents uneven sections

A

Clearance angle

24
Q

Specimen is transferred from container to container for 16 hours

A

Tissue transfer (dip & dunk)

25
Q

Tissues remain in one container but the reagent changes at intervals

A

Enclosed type tissue processor

26
Q

Wax bath thermostat should be at least ___ the melting point of the wax

A

3° above

27
Q

its the mechanically raising and lowering of the tissue into reagent containers providing agitation needed

A

Vertical oscillation

28
Q

Stations 1-2 of autotechnicon

A

Fixation w/ 10% formalin

29
Q

Stations 3-6 of autotechnicon

A

Dehydration w/ ascending ethyl alcohol 70-95%

30
Q

Stations 7-8 of autotechnicon

A

2 changes of acetone

31
Q

Stations 9-10 of autotechnicon

A

Clearing with 2 changes of chloroform/xylol

32
Q

Stations 11-12 of autotechnicon

A

Impregnation w/ 2 changes of liquid paraffin

33
Q

Temperature for floatation water bath

A

45-50°C

or 6-10°C lower than the melting point of wax used

34
Q

squamous cell contaminants from ungloved fingers

A

Floaters

35
Q

Wax impregnation under negative atmospheric inside an embedding oven & gives fastest result

A

Vacuum embedding apparatus

36
Q

Temperature of drying oven

A

Infiltration process: 55-60°C
Drying process: 56-60°C for 2hrs

37
Q

Glass slides for routine work
size:
refractive index:

A

76 x 25 mm with 1-1.2 mm thick (frosted)
1.518

38
Q

Labelling in histopathology

A

Foolproof

HISTO (8 decks)

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