Fresh tissue examination

Question 1

Simplest & least invasive
using 25g 10cc

Answer 1

Fine needle aspiration

Question 2

Removes cells & small amount of the surrounding tissues

Answer 2

Core needle biopsy

Question 3

Takes out more surrounding tissue & abnormality but not all

Answer 3

Incisional biopsy

Question 4

Removing the entire area in question

Answer 4

Excisional biopsy

Question 5

Used in obtaining full-thickness skin spx using a circular blade (3-4mm cylindrical core)

Answer 5

Punch biopsy

Question 6

Small fragments are removed from a surface

Answer 6

Shave biopsy

Question 7

Tissue is scooped/spooned to remove growths in endometrium/cervical canal

Answer 7

Curettings

Question 8

specimen is immersed in a watch glass w/ isotonic salt soln

Answer 8

Teasing/Dissociation

Question 9

Pieces of specimen are forcibly compressed with another slide w/ a cover glass

Answer 9

Squash prep (< 1mm)

Question 10

Used in cytological exams

Answer 10

smear prep

Question 11

method where secretions are uniformly distributed & directly applied on the slide

Answer 11

streaking

Question 12

method where mucous is gently spread into a moderately thick film

Answer 12

spreading

Question 13

method where FNAB specimens are dispersed evenly over the surface of the 2 slides

Answer 13

Pull-apart

Question 14

A surface of a freshly cut piece of tissue & pressed on the surface of a clean slide w/o destroying their intercellular relationship

Answer 14

Touch prep (impression smear)

Question 15

Commonly used in
- rapid patho dx
- enzyme histochemistry
- dx of lipids & carbs
- immunofluorescent & immunohistochemical staining
- neuropatho

Answer 15

Frozen sections

Question 16

slow freezing can cause distortion due to

Answer 16

Ice crystal artifacts

Question 17

Methods used for freezing & which is the most rapid among them

Answer 17

liquid nitrogen (most rapid)
isopentane cooled by liq. nitrogen
CO2 gas
Aerosol sprays

Question 18

Procedure where different temps between tissue & knife are used
CO2 gas used

Answer 18

Cold knife procedure

Question 19

Optimum condition for cold knife
Knife:
Tissue:
Environment:

Answer 19

Knife: -40 to -60°C
Tissue: -5 to -10°C
Environment: 0 to - 5°C

Question 20

Procedure where sections are cut in isothermic condition (____)
insulated rotary microtome

Answer 20

Cryostat (-18 to -20°C)

Question 21

Temp of cryostat cold chamber

Answer 21

-5 to -30°C

Question 22

Conventional tissue processing

Answer 22

Fixation
Decalcification (optional)
Dehydration
Clearing
Impregnation
Embedding (casting/blocking)
Trimming
Sectioning
Staining
Mounting
Labelling