Inhibition of oxidative phosphorylation

Question 1

What complex does Rotenone and Amytal (pesticide, insecticide and non-selective piscicide) inhibit

Answer 1

Complex 1

Question 2

What complex doe Antimycin A (Streptomyces antibiotic) inhibit

Answer 2

Complex III

Question 3

What complex does Carbon monoxide and cyanide (e- transported to oxygen) inhibit

Answer 3

Complex IV

Question 4

What complex is most dangerous to inhibit

Answer 4

Complex IV, because blocking the entire chain is the most drastic effect (cant transport e- onto oxygen)

Question 5

What complex is least dangerous to inhibit

Answer 5

complex I, still can bypass using complex II (FADH) or glycerol-3-phosphate transporter

Question 5

What complex is least dangerous to inhibit

Answer 5

complex I, still can bypass using complex II (FADH)

Question 6

How many ATP are produced if oxidation phosphorylation is inhibited by Rotenone

Answer 6

7 ATP

Question 7

How many ATP are produced if we use the glycerol shuttle and inhibit with Rotenone

Answer 7

10 ATP

Question 8

How many ATP are produced if we use the glycerol or malate shuttle and inhibit with CO

Answer 8

4