Inflammation 2 Flashcards
Sequence of leukocyte events
1.Margination, rolling, adhesion
2.Transmigration across endothelium (also called diapedesis)
3.Migration in interstitial tissues toward chemotactic stimulus (chemotaxis)
4.Phagocytosis and synthesis of biochemical mediators
Neutrophil margination
-vasodilation results in slower flow of blood which allows the neutrophils to move to the edges
-edema and stasis
Neutrophil rolling
-binding of proteins on endothelium and the neutrophils
>done by selectins. Slows the neutrophils and rolls them along the endothelium
Neutrophil Adhesion
-getting the neutrophils to stop and have firm adhesion is conducted by integrins (beta1 and beta2)
Leukocyte adhesion deficiencies
-Cattle, dogs (irish setters) and people with Leukocyte adhesion disorders that lack the expression of beta 2 integrins
>means neutrophils can slow down, but never stop and can’t get into site of infection. Therefore neutrophils high in circulation but low numbers in sites of infection
-animals typically die within a few days/weeks
BLAD
-cattle leukocyte adhesion deficiency
-develop severe gingivitis, tooth loss, oral ulcers, enteric ulcers, cutaneous ulcers, diarrhea, pneumonia (**note all things that are interacting with the outside environment)
Leukocyte types depending on time
-Early inflammation: neutrophils because there is many of them, they respond rapidly to cytokines, and they adhere strongly to adhesion molecules. But they have a short lifespan once they leave the circulatory system
-Late inflammation: neutrophils are replaced by monocytes (long life)
Viral infections
-lymphocytes predominant
Hypersensitivity reactions
-eosinophils predominant
Chemotaxis
-directed movement of cells toward a chemical attractant. They will move toward the higher amount/gradient
Hydrocephalis
-congenital or acquired
>congenital if the skull is dome shaped because no way for the skull to change afterwards
-enlarged ventricle, very large brain and dome shaped skull
-excess fluid in the brain
Leukocyte induced injury
-Eg. pus filled kidney
>leukocytes release lysosomal substances into extracellular space= Frustrated phagocytosis
Frustrated phagocytosis
-blob attached to something and neutrophils are unable to properly engulf
-when it can’t fully surround, the neutrophils will “barf” everything out (lysozymes) and destroy surrounding tissues by accident
Smoke induced frustrated phagocytosis
-Smoking results in antigens that result in neutrophils releasing elastase through frustrated phagocytosis which breaks down elastin in the alveoli, resulting in smoke induced pulmonary emphysema
Heave line in horse
-Horse is working heard to breath
-results from chronic inflammatory response to lung
Neutrophils release Reactive oxygen species
-results in damage to surrounding structures
Neutrophils are indiscriminate about what they target
**reason why you would want to minimize immune response
-occurs through NETs, reactive oxygen species, lysozymes
Mandible of Cow
-pylogranulomas inflammation in jaw resulting in lumpy jaw, chronic legion
>morphologic diagnosis: abscesses or bone growth