Diagnostic oncology

Question 1

Methods to diagnose tumours

Answer 1

-Histopathology (H&E staining)
-Histochemical stains (Toluidine blue, or Duffy’s stain)
-Immunohistochemistry (expression of antigens helps identify cell of origin)
-Clonality Assays (PCR for receptor rearrangement)

Question 2

Tumour grading

Answer 2

-attempt to sub classify tumours of similar cellular origin to better determine prognosis
>Mast cells OR soft tissue sarcomas. Can range from serious to just removal and animal will be fine.

Question 3

Tumour grading systems

Answer 3

-when people are indecisive, they place everything into grade 2
**issues with consistency

Question 4

Soft tissue sarcoma

Question 5

Tumour staging

Answer 5

-method of indicating clinical spread of tumour. Describe what the tumour is doing:
>TMN
»Size of tumour (T)
»Lymph node involvement (N)
»Presence of metastasis (M)

Question 6

Assessment of tumour margins

Answer 6

-excisional/incisional biopsies
-histologic evaluation of margins of biopsy specimens

Question 7

Excisional biopsy

Answer 7

-take mass and a larger portion around it

Question 8

Incisional biopsy

Answer 8

-take a slice/portion of the mass
-will get it tested and then figure out what to do