Infections

Question 1

Differences between DNA&RNA virues

Answer 1

DNA-no V-onc- tranformation only happens in non permissive

RNA- V onc- can intergrate

Question 2

mouse polyamomavrius- how do they deregulate

Answer 2

DNA virus
non permissive hosts
Early genes(Large, Middle and Small T)
stimulate cell growth and rarely intergrated
Virus cannot replicate
Late genes(VP1,2,3) not intergrate-cannot regulate

Question 3

MPV- 2 stage Cancer discovery

Answer 3

Rat fibroblasts
Large T+ MiddleT= tranformation/ cancerous
Middle T alone= tranformation of immortalised fib
Large T= immortalisation

Question 4

Mechanisms of middle T

Answer 4

cell membrane, stimulates RAS,AKT,PI3K pathway- prolferation

Question 5

Mechanisms of Large T (SV40)

Answer 5

Inhbits Rb pathway- LxCxE domain
Inactivates P53- interaction with bipartite region
Ali(2001)

Question 6

How did the fibroblast system help define oncogenes?

Answer 6

Immortalisation genes- nuclues protein- deregulation at DNA levels
transformatation genes- Plasma membrane- stimulated growth responses.