industrial biotechnology Flashcards
what is genetic engineering?
developing procdures for isolation and manipluation of genetic material
what is an example of genetic engineering?
insulin production
what is molecular cloning?
it is the isolation and purification of a single vector
turns a plasmid into a closing vector
why are plasmids good for cloning?
smaller, circular, stable, independent orgin, multiple copy number
what is a good host for a cloning vector?
rapid growth, not harmful, stable in culture, can grow in inexpensive medium, have right enzymes to allow for replication, can take up DNA
what are 8 ways to genetic engineer?
- microbial fermentation
- virus vaccines
- mammaliam protiens
- transgenic plants and animals
- environmental biotechnology
- gene regulation and gene therapy
- bleaching
- soil recovery
what is in the production of inulin?
polypeptides a and B by disulfide bridges
what does pseudomonas syringe do?
makes biofilm and grows very readily and some forms are ice plus and have a ice nucleating protein which super cools
what is ice nucleation?
it is damage to the membrane water is supercooled to act has a catalyst
=less frost damage
recovery of metals?
is used for low grade copper and uranium ores
this process can be recycled
anaerobic prokaryotes
re oxidize NADH by respiration or fermentation