IMSE PRAC LAB Flashcards
Most common and ubiquitous pathogenic bacteria
Streptococcus pyogenes
Major virulence factor of group A streptococcus
M protein
Responsible for rash seen in Scarlet fever
Pyrogenic exotoxins A, B, C
Signs and symptoms of scarlet fever
Fine red rash
white coating on toungue
swollen glands on neck
fever over 38.3°C
Painful joints
Conditions of increased ASO (5)
Scarlet fever
Acute glomerulonephritis
Acute rheumatic fever
Necrotizing fasciitis
Streptococcal toxic shock syndrome
Scarlet fever is caused by
Group A Streptococcus
Principle of ASO slide method
Passive or indirect agglutination
Specimen or sample used in ASO slide method
serum
ASO latex reagent used in ASO slide method
Suspension of polystyrene particles sensitized with streptolysin O
Sensitivity of a ASO slide method
200 IU/mL
Reagent used in a ASO positive control
Stabilized human room containing ASO reactive with ASO latex reagent
Reagent used in a ASO negative control
Stabilized human serum containing ASO non-reactive with ASO latex reagent
Preservative of all the reagents
0.1% sodium azide
Measures antibodies in serum of patient with typhoid fever
Widal test
3 kinds of antigens
O antigen
H antigen
K antigen
Other name of O antigen
Somatic antigen
Other name of H antigen
Flagellar antigen
Other name of K antigen
Vi antigen
3 types of widal’s test
Rapid slide screening method
Rapid slide titration method
Tube agglutination test
Used to help diagnose typhoid fever
Widal test
2 bacteria detects in widal test
Salmonella enterica
Salmonella typhi
Volume of saline solution in each tubes
0.25 mL
What pipette use in serial dilutions?
serological pipette
Volume of antibody A
0.25 mL
Volume of 3% red blood cell suspension
0.25 mL
Refrigeration time for the test tubes
30 minutes
Centrifuge the tubes for what time?
20 seconds
Last tube showing agglutination is called
Endpoint
Last dilution showing a positive result is called
Titer
Formula of simple dilution
Amount of solute / Total volume
