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ASO DETERMINATION Flashcards

1
Q

STREPTOCOCCI IS DIVIDED INTO: (2)

A

GROUPS & SEROTYPES/SEROGROUP

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2
Q

GRAM POS
SPHERICAL
OVOID
CATALASE NEG
SEEN IN PAIRS OR CHAINS

A

STREPTOCOCCI

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3
Q

OUTERMOST CELL WALL COMPONENTS

A

SEROTYPE/SEROGROUP

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4
Q

2 MAJOR PROTEINS IN SEROTYPE/SEROGROUP

A

M PROTEIN

T PROTEIN

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5
Q

DETERMINE THE SEROTYPE OF THE STREPTOCOCCUS SPP.

A

M PROTEIN & T PROTEIN

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6
Q

DIVIDES STREPTOCOCCI INTO 20 DEFINED GROUPS A-H & K-V

A

GROUP SPECIFIC CARBOHYDRATE

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7
Q

WHAT ARE THE 5 EXOANTIGENS

A

DNase B
NADase
STREPTOKINASE
HYALURONIDASE
STREPTOLYSIN O

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8
Q

MOST COMMON AND UBIQUITOUS PATHOGENIC BACTERIA

A

STREPTOCOCCUS PYOGENES

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9
Q

MAJOR VIRULENCE FACTOR OF S. pyogenes

A

M PROTEIN

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10
Q

ADDITIONAL VIRULENCE FACTOR EXCRETED BY BACTERIAL CELLS (1)

A

EXOANTIGENS / EXOTOXIN

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11
Q

RESPONSIBLE FOR RASH SEEN IN SCARLET FEVER AND CONTRIBUTE TO PATHOGENICITY

A

PYROGENIC A, B,C

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12
Q

MOST DIAGNOSTICALLY IMPORTANT ANTIBODIES (4) AND AN ANTIBODIES INDICATION OF GROUP A STREPTOCOCCUS INFECTION

A

ANTI-DNase B
ANTI-NADase
ANTI-hyaluronidase
ANTI- streptolysin O

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13
Q

CONDITIONS OR DISEASE SEEN IN ANTIBODIES OF GROUP A STREPTOCOCCUS (5)

A

SCARLET FEVER

ACUTE RHEUMATIC FEVER

ACUTE GLOMERULONEPHRITIS

NECROTZIING FASCIITIS

STREPTOCOCCAL TOXIC SHOCK SYNDROME

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14
Q

HOW TO SPOT SCARLET FEVER OR SYMPTOMS OF SCARLET FEVER (8)

A

FINE RED RASH, FEELS LIKE SANDPAPER

STRAWBERRY TONGUE

FEVER OVER 38.3 C or 101 F

FLUSHED RED FACE BUT PALE AROUND MOUTH

SWOLLEN GLANDS ON NECK

PAINFUL JOINTS

ERYTHEMA MARGINATUM

NARROWED MITRAL VALVE

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15
Q

ASO AGGLUTINATION REACTION (6)

A

DIRECT AGGLUTINATION

PASSIVE AGGLUTINATION

REVERSE PASSIVE AGGLUTINATION

AGGLUTINATION INHIBITION

AHG-MEDIATED AGGLUTINATION (COOMB’S TEST)

COAAGGLUTINATION

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16
Q

DIRECT COOMBS TEST (3)

A

HEMOLYTIC DISEASE ON THE NEWBORN (HDN)

HEMOLYTIC TRANSFUSION REACTION (HTR)

AUTOIMMUNE HEMOLYTIC ANEMIA (AIHA)

17
Q

INDIRECT COOMBS TEST (4)

A

CROSS-MATCHING

ANTIBODY DETECTION

ANTIBODY IDENTIFICATION

RBC ANTIGEN PHENOTYPING

18
Q

PRINCIPLE OF ASO SLIDE METHOD

A

PASSIVE/INDIRECT AGGLUTINATION

19
Q

SPECIMEN/SAMPLE USED IN ASO SLIDE METHOD

20
Q

ASO LATEX REAGENT USED

A

SUSPENSION OF POLYSTYRENE LATEX PARTICLES SENSITIZED WITH STREPTOLYSIN O

21
Q

ANALYTICAL SENSITIVITY OF ASO SLIDE METHOD:

NORMAL:
POSITIVE:
NEGATIVE:

A

NORMAL: 200 IU/mL
POSITIVE: TITER IS > 200 IU/mL
NEGATIVE: TITER IS < 200 IU/mL

22
Q

REAGENT USED IN ASO POSITIVE CONTROL

A

STABILIZED HUMAN SERUM CONTAINING ASO REACTIVE WITH ASO LATEX REAGENT

23
Q

REAGENT USED IN ASO NEGATIVE CONTROL

A

STABILIZED HUMAN SERUM CONTAINING ASO NON REACTIVE WITH ASO LATEX REAGENT

24
Q

ALL REAGENT OF ASO CONTAINS AND ACT AS PRESERVATIVE

A

0.1% SODIUM AZIDE

25
Q

ASO PROCEDURE FOR QUALITATIVE METHOD

A
  1. ALLOW EACH REAGENT TO REACH ROOM TEMPERATURE
  2. GENTLY MIX THE REAGENT TO EVENLY DISTRIBUTE LATEX PARTICLES
  3. PLACE A DROP OF UNDILUTED SERUM INTO A CIRCLE OF TEST SLIDE
  4. ADD 1 DROP OF ASO LATEX REAGENT NEXT TO THE DROP OF SERUM
  5. MIX GENTLY USING STIRRER
  6. GENTLY TILT THE SLIDE BACK AND FORTH FOR 2 MINUTES
26
Q

POSITIVE RESULT IN ASO SLIDE

A

AGGLUTINATION

27
Q

NEGATIVE RESULT IN ASO SLIDE

A

NO AGGLUTINATION

28
Q

INTERFERENCES OF ASO SLIDE QUALITATIVE METHOD (3)

A

BILIRUBIN 20mg/dl

HEMOGLOBIN 10g/l

LIPIDS 10g/l

29
Q

REFERENCE RANGE OF ASO SLIDE QUALITATIVE METHOD

ADULTS:
CHILDREN <5:

A

ADULTS: UP TO 200 IU/mL

CHILDREN <5: 100 IU/mL

30
Q

ASO PROCEDURE FOR SEMI QUANTITATIVE METHOD

DILUTIONS:
SAMPLE SERUM VOLUME:
SALINE VOLUME:
VOLUME OF SAMPLE:
DILUTION FACTOR:
CONCENTRATION:

A

DILUTIONS: 1/2,1/4, 1/8, 1/16, 1/32
SAMPLE SERUM VOLUME: 100 ul for 1/2 only
SALINE VOLUME: ALL 100 ul
VOLUME OF SAMPLE: ALL 50 ul
DILUTION FACTOR: 200, ex. 1/4—>200x4
CONCENTRATION: 400 IU/mL, 800 IU/mL, 1600 IU/mL, 3200 IU/mL &
6400 IU/mL

31
Q

NORMAL LEVELS OF ASO SEMI QUANTITATIVE METHOD

A

<200 IU/mL

32
Q

IF AN AGGLUTINATION INVOLVES RED BLOOD CELL IT IS CALLED

A

HEMAGGLUTINATION

33
Q

OCCURS WHEN ANTIGENS FOUND NATURALLY ON A PARTICLE

A

DIRECT AGGLUTINATION

34
Q

EMPLOYS A PARTICLE THAT ARE COATED WITH ANTIGENS

A

PASSIVE AGGLUTINATION

35
Q

EMPLOYS A PARTICLE THAT ARE COATED WITH ANTIBODIES

A

REVERSE PASSIVE AGGLUTINATION

36
Q

COMPETITION BETWEEN PARTICULATE AND SOLUBLE ANTIGENS FOR LIMITED ANTIBODY COMBINING

CLASSIC PREGNANCY AGGLUTINATION REACTION

A

AGGLUTINATION INHIBITION

37
Q

USES BACTERIA AS THE INERT PARTICLES TO WHICH ANTIBODY IS ATTACHED

A

COAAGGLUTINATION

38
Q

DETECTS NON AGGLUTINATING ANTIBODY

A

COOMB’S TEST

IMSE (5 decks)

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