Important equations Flashcards
Henderson - HasselBach
[A-]/[HA] = 10^pH -pKa
Fraction deprotonated
x = (10^pH-pKa)/(1+10^pH-pKa)
Absorbance equations (2)
A= log(Io/I)
A=εcl
Enzyme unit
amount of enzyme that catalyses 1μmol of substrate in one minute at 25 C
Specific activity
Enzyme units / total protein (mg)
Fraction occupied
θ=[PL]/([PL]+[P])
dissociation constant
Kd = [P][L]/[PL]
O2 binding fraction
θ = [O2] / ([O2] + Kd)
= pO2 /
pO2 + p50
How to find Hill Coefficient (nH)
slope of log(θ/(1-Θ) vs log(pO2)
Michaelis constant
Km = (k-1+k2)/k1
[ES] as function of Km
[ES] = [Et][S] / (Km+[S])
Michaelis Menten Equation
Vo = (Vmax[E]/ (Km = [S])
catalytic efficiency
kcat / Km
LinewraverBurk equation
1/Vo = (αKm/Vmax)(1/[S]) + (α’/Vmax)
α
α = 1+ [I]/Ki