Important equations Flashcards

1
Q

Henderson - HasselBach

A

[A-]/[HA] = 10^pH -pKa

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2
Q

Fraction deprotonated

A

x = (10^pH-pKa)/(1+10^pH-pKa)

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3
Q

Absorbance equations (2)

A

A= log(Io/I)

A=εcl

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4
Q

Enzyme unit

A

amount of enzyme that catalyses 1μmol of substrate in one minute at 25 C

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5
Q

Specific activity

A

Enzyme units / total protein (mg)

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6
Q

Fraction occupied

A

θ=[PL]/([PL]+[P])

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7
Q

dissociation constant

A

Kd = [P][L]/[PL]

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8
Q

O2 binding fraction

A

θ = [O2] / ([O2] + Kd)

= pO2 /
pO2 + p50

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9
Q

How to find Hill Coefficient (nH)

A

slope of log(θ/(1-Θ) vs log(pO2)

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10
Q

Michaelis constant

A

Km = (k-1+k2)/k1

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11
Q

[ES] as function of Km

A

[ES] = [Et][S] / (Km+[S])

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12
Q

Michaelis Menten Equation

A

Vo = (Vmax[E]/ (Km = [S])

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13
Q

catalytic efficiency

A

kcat / Km

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14
Q

LinewraverBurk equation

A

1/Vo = (αKm/Vmax)(1/[S]) + (α’/Vmax)

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15
Q

α

A

α = 1+ [I]/Ki

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16
Q

α’

A

α’ = 1 + [I]/K’i