Immunisation and Prophylaxis Flashcards
What are the different types of vaccines
Live attenuated Inactivated (killed) Toxoid vaccine Subunit of micro-organism - purified microbial product - recombinant
Examples of live attenuated vaccines
MMR BCG Varicella-zoster virus Yellow fever Smallpox
Examples of Inactivate vaccines
Poli HBA Cholera (oral) Rabies Japanese Encephalitis Tick-borne encephalitis Influenza
What is herd immunity
The resistance to the spread of a contagious disease within a population that results in a sufficiently high proportion of individual being immune to the disease (commonly through vaccination)
I.e because so many people are immunised those who are contagious are unlikely to come into contact with someone who is not immunised
What type of vaccine is HBV
Recombinant
What is a live attenuated vaccine?
Vaccines which use a weekend/attenuated form of the organism which causes the disease
Beware in those who are immunocompromised
What are inactivated vaccines
Used the killed (inactivated) form of the micro-organism which causes the disease
What is a Toxoid vaccine
Use a toxin from the organism and treat it with formula to make a toxoid
Give an example of a toxoid vaccine
Diptheria
Tetanus
What is a recombinant vaccine
A recombinant vaccine is a vaccine produced through recombinant DNA technology. This involves inserting the DNA encoding an antigen (such as a bacterial surface protein) that stimulates an immune response into bacterial or mammalian cells, expressing the antigen in these cells and then purifying it from them.
What creates the stronger and faster response in secondary response?
Immunological memory
2 ways in which we can gain adaptive immunity
Though natural exposure
Or
Through artificial exposure
What 2 natural ways can we gain adaptive immunity
Passive (from maternal)
Active (infection)
What are the 2 artificial ways in which we can gain adaptive immunity
Passive (transfer of antibodies - someone else antibodies)
Active (immunisation - we make our own antibodies)
Describe the steps to making the HBV vaccine
DNA segment coding for HBsAg is removed from HBV
It is then purified and mixed with plasmids from bacterial cells
This new genetically enigineered plasmid is then inserted into yeast cells
These yeast cells producing the HBV antibodies are then grown
The HBsAg is produced
This is then removed from the yeast cells and used to create the HBV vaccine