Imaging of Systems

Question 1

How do you preserve embed and stain for light microscopy?

Answer 1

Formalin
Paraffin
H&E

Question 2

How do you cut preparations? What happens to the specimen to help make it easier to cut?

Answer 2

Microtome

Freeze it

Question 3

What colours are H, E and H&E staining?

Answer 3

Blue, Pink, Purple

Question 4

Confocal microscopy resembles which imagine commonly used on humans?

Answer 4

CT

Question 5

Immunofluorescence microscopy - type of immunohistochemistry

Answer 5

Uses an antibody e.g. monoclonal with fluorescence to detect a protein e.g. tubulin

Question 6

Autoradiography looks like what?

Answer 6

Black and white photograph

Question 7

Whats TEM (transmission electron microscopy). Is it B&W or colour

Answer 7

Uses electrons, higher magnification than light microscopy, electrons pass through tissue, those that absorb electrons appear darker, those that let electrons through appear bright. B&W

Question 8

NMR (nuclear magnetic resonance spectroscopy) is what

Answer 8

gives electronic structure of molecule by measuring the magnetic field of a molecule/atom via change in resonant frequency