II. STREPTOCOCCI Flashcards
TRUE OR FALSE:
Streptococci are commonly found as part of normal human flora.
TRUE. However, when these organisms access to normally sterile sites, they can cause life threatening infection.
Microscopy of Streptococci:
grayish, translucent to slightly opaque colonies; mucoid colonies
Growth of streptococci is enhanced by:
blood, serum, or glucose incorporated in agar plate
Grows in the presence of oxygen but unable to use oxygen for respiration:
aerotolerant anaerobes
Notorious pathogens:
S. pyogenes and S. pneumoniae
Classifications of Streptococci:
- Academic/Bergey’s Classification
- Smith and Brown Classification
- Lancefield Classification
Classification based in temperature requirement:
Academic/Bergey’s classification
Groups under Academic Classification:
- pyogenic group
- viridians group
- lactic group
- enterococcus group
Grows only at 37C:
pyogenic group
It produces pus; mostly B-hemolytic:
pyogenic group
Species under pyogenic group:
S. pyogenes
Grows at both 45C and 37C:
viridians group
It is not part of the Lancefield group; may be alpha hemolytic or nonhemolytic:
viridians group
Normal biota in the URT in humans:
viridians group
Species under viridians group:
“SM Mandaue”
S. salivarius
S. mutans
S. mitis
Grows on 10C and 37C:
lactic group
Often found in dairy products:
lactic group
Species under lactic group:
S. lactis
Causes normal coagulation or souring of milk:
S. lactis
Grows at 10C, 45C, and 37C:
enterococcus group
Normal flora of human intestine:
enterococcus group
Species under enterococcus group:
E. faecalis
Classification based on hemolytic patterns:
Smith and Brown Classification
Types of hemolytic pattern under Smith and Brown Classification:
- alpha-hemolytic streptococci
- beta-hemolytic streptococci
- gamma-hemolytic streptococci
Have partial/incomplete hemolysis; green hemolysis:
alpha-hemolytic streptococci
Species under alpha-hemolytic streptococci:
S. pneumoniae (green streptococci)
Exhibit complete lysis of RBCs; clear area/zone:
beta-hemolytic streptococci
Species under beta-hemolytic streptococci:
“PA DA”
S. pyogenes
S. agalactiae
S. dysagalactiae subsp. equisimilis
S. anginosus group
No lysis or RBCs:
gamma-hmeolytic streptococci
Species under gamma-hemolytic streptococci:
E. faecalis
Classification based on the extraction of C carbohydrate from the streptococcal cell wall:
Lancefield Classification (Antigen Serogrouping)
Founds out that the C carbohydrate can be extracted by putting the organism in dilute acid and heating for 10 minutes:
Rebecca Lancefield
Lancefield Classification is mostly significant in classifying and identifying:
B-hemolytic streptococci
Species under Group A:
S. pyogenes
Species under Group B:
S. agalactiae
Species under Group D:
enterococci
Species not included in the Lancefield Classification:
S.pneumoniae and Viridans group streptococci