GROUP B, C, and G STREPTOCOCCI

Question 1

It is nosocomially transmitted by unwashed hands of mother or healthcare personnel to the newborn:

Answer 1

Group B streptococci

Question 2

Normal flora of the female genital tract and lower GIT:

Answer 2

Group B streptococci

Question 3

Recommended time for pregnant women to be screened for group B streptococci:

Answer 3

35-37 weeks of gestation

Question 4

Species under Group B streptococci:

Answer 4

S. agalactiae

Question 5

Culture of Group B streptococci:

Answer 5

grayish white, mucoid colonies; small zone of B-hemolysis

Question 6

Virulence factor:

Answer 6

capsule

Question 7

Significant component of the capsule:

Answer 7

sialic acid

Question 8

Avirulent factors:

Answer 8

1. hemolysin
2. CAMP factor
3. neuraminidase
4. deoxyribonuclease
5. hyaluronidase
6. protease

Question 9

Diseases/Infections:

Answer 9

“NOPE PUM”
Neonatal sepsis
Osteomyelitis
Pneumonia
Endocarditis
Postpartum infection
UTI
Meningitis

Question 10

Differentiate S. agalactiae from other B-hemolytic streptococci:

Answer 10

CAMP test and hippurate hydrolysis test

Question 11

CAMP test stands for:

Answer 11

Christie, Atkins, Munch, Peterson

Question 12

Enhances B-hemolytic activity:

Answer 12

CAMP factor

Question 13

Reagent of CAMP test:

Answer 13

B-lysin producing strain of S. aureus or B-lysin disk

Question 14

CAMP test (+) result:

Answer 14

arrow-head B-hemolysis or bowtie appearance

Question 15

Cultivation media use for CAMP test:

Answer 15

1. Blood agar: beta-hemolytic
2. Todd-Hewitt Broth: contain antimicrobials which suppresses the growth of GBS
3. LIM Broth

Question 16

Reagent of Hippurate Hydrolysis test:

Answer 16

sodium hippurate + ninhydrin

Question 17

Hippurate Hydrolysis test (+) result:

Answer 17

purple color after adding ninhydrin reagent (indicates hippurate hydrolysis)

Question 18

Hippurate Hydrolysis test (-) result:

Answer 18

no color change

Question 19

S. agalactiae is resistant to:

Answer 19

bacitracin and SXT

Question 20

Recovered from URT, vagina and skin of humans:

Answer 20

Group C and G streptococci

Question 21

Main source of streptokinase:

Answer 21

Group C

Question 22

Species under Group C and G:

Answer 22

“DeEz” (nutz)
S. dysagalactiae subsp. equisimilis
S. equi subsp. zooepdemicus

Question 23

Antimicrobial susceptibility test for Group C:

Answer 23

susceptible to bacitracin and SXT

Question 24

Antimicrobial susceptibility test for Group G:

Answer 24

bacitracin resistant or susceptible

Question 25

2 Forms of Group C and G streptococci:

Answer 25

1. large-colony forming isolates
2. small-colony forming isolates

Question 26

Large-colony-forming isolates with groups A, C, and G antigens are classified as:

Answer 26

pyogenic streptococci

Question 27

Large-colony-forming B-hemolytic isolates with groups C and G antigens belongs to:

Answer 27

S. dysagalactiae subsp. equisimilis

Question 28

Small-colony-forming B-hemolytic isolates with groups C and G antigens belongs to:

Answer 28

S. anginosus group (included in the viridians group)