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MYCOLOGY AND VIROLOGY > IDENTIFICATION OF SEVERAL FUNGAL CULTURE MEDIA > Flashcards

IDENTIFICATION OF SEVERAL FUNGAL CULTURE MEDIA Flashcards

1
Q

Brain-heart infusion agar composition. Primary recovery of saprobic and pathogenic fungi

A

1.Brain-heart infusion
2.enzymatic digest of animal tissue,
3.enzymatic digest of casein,
4.dextrose
5. sodium chloride

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2
Q

Brain-heart infusion agar with antibiotics composition. Primary recovery of pathogenic fungi exclusive of dermatophytes

A

1.Brain-heart infusion
2.enzymatic digest of animal tissue,
3.enzymatic digest of casein,
4.dextrose,
5.sodium chloride,
6.antibiotics

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3
Q

Brain-heart infusion biphasic blood culture
bottles. Recovery of fungi from blood

A

1.Brain-heart infusion
2.peptone
3.glucose
4.disodium phosphate

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4
Q

Isolation and presumptive
identification of yeast and filamentous fungi

A

Chromogenic agar

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5
Q

Chromogenic agar composition:

A

Chromopeptone
Glucose
Chromogen mix
Chloramphenicol

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6
Q

Primary recovery of dermatophytes;
recommended as screening medium

A

Dermatophyte test medium

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7
Q

Dermatophyte test medium composition:

A

1.Dextrose
2.cycloheximide,
3.gentamycin
4.chloramphenicol
5.phenol red

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7
Q

Inhibitory mold agar composition. Primary recovery of pathogenic fungi exclusive of
dermatophytes

A

Chloramphenicol,
casein,
dextrose,
starch,
sodium phosphate,
magnesium sulfate,
sodium chloride,
manganese sulfate

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8
Q

Potato flake agar composition. Primary recovery of saprobic and pathogenic
fungi

A

Potato flakes
glucose,
cycloheximide,
chloramphenicol,
bromthymol blue

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9
Q

Mycosel agar COMPOSITION Primary recovery of dermatophytes

A

Cycloheximide,
chloramphenicol,
dextrose

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10
Q

Sabouraud Dextrose with Brain Heart
Infusion (SABHI) agar. Primary recovery of
saprobic and pathogenic fungi

A

Sabouraud dextrose
brain-heart infusion agar

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11
Q

Yeast-extract phosphate agar. Primary recovery of pathogenic fungi
exclusive of dermatophytes

A

Yeast extract,
dipotassium phosphate,
chloramphenicol

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12
Q

DIFFERENTIAL TEST MEDIA
Identification of Cryptococcus, Trichosporon, and Rhodotorula spp.

A

Christensen’s urea agar

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13
Q

Christensen’s urea agar composition

A

2% urea, phenol red

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14
Q

Identification of Candida albicans by
chlamydospore production Identification of C.
albicans by microscopic morphology

A

Cornmeal agar with Tween 80 and trypan
blue

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15
Q

Cornmeal agar with Tween 80 and trypan
blue composition

A

Cornmeal, Tween 80, trypan blue

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16
Q

Conversion of the dimorphic fungus
Blastomyces spp. from mold to yeast form

A

Cottonseed conversion agar

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17
Q

Cottonseed conversion agar composition

A

Cottonseed meal, glucose

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17
Q

Differential identification of Aspergillus spp.

A

Czapek’s agar

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18
Q

Czapek’s agar composition:

A

Sodium nitrate
sucrose
yeast extract

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19
Q

Identification of Cryptococcus
neoformans and Cryptococcus gattii

A

Niger seed agar (birdseed agar)

20
Q

Niger seed agar (birdseed agar) composition:

A

Guizotia abyssinica seed,
dextrose,
chloramphenicol

21
Q

Detection of nitrate reduction to confirm
Cryptococcus spp.

A

Nitrate reduction
medium

22
Q

Nitrate reduction medium composition:

A

Potassium nitrate
peptone
meal extract
sulfanilic acid,
N-dimethyl-1-naphthylamine

23
Q

Demonstration of pigment production by
Trichophyton rubrum Preparation of microslide cultures and sporulation
of dermatophytes

A

Potato dextrose agar

24
Q

Potato dextrose agar composition

A

Potato infusion, D(+) glucose

25
Q

Identification of Microsporum audouinii

A

Rice medium

26
Q

Rice medium composition:

A

White rice extract
polysorbate 80

27
Q

Identification of Trichophyton spp.

A

Trichophyton agars 1-7

28
Q

Trichophyton agars 1-7 composition:

A

Casamino acids
dextrose
monopotassium phosphate,
magnesium sulfate
amino acids,
ammonium nitrate

29
Q

Casamino acids, dextrose,
monopotassium phosphate,
magnesium sulfate, amino acids,
ammonium nitrate

A

Trichophyton agars 1-7

30
Q

Urea agar composition:

A

Peptone,
dextrose,
sodium chloride,
monopotassium phosphate,
urea,
phenol red

31
Q

Identification of yeasts by determining
fermentation

A

Yeast fermentation
broth

32
Q

Yeast fermentation broth composition

A

Yeast extract,
peptone,
bromcresol purple
specific carbohydrate

33
Q

Identification of yeasts by determining
carbohydrate assimilation

A

Yeast nitrogen-base agar

34
Q

Yeast nitrogen-base agar composition

A

Ammonium sulfate
carbon source

35
Q

This important procedure often can provide the first microbiologic proof of the cause of disease in patients with fungal infection and guide the selection of appropriate media to
support growth.

A

Direct Microscopic Examination

36
Q

Detection of Mycobacteria, Nocardia spp. and some isolates of Blastomyces spp.

A

Acid-fast stain and partial acid-fast stain

37
Q

● Detection of Mycobacteria and Nocardia
● Excellent screening tool; sensitive and affordable.

A

Auramine-rhodamine stain

38
Q

Detects fungi rapidly because of bright fluorescence.

A

Calcofluor white stain

39
Q

Commonly performed on most clinical specimens submitted for bacteriology; detects most fungi.

A

Gram stain

40
Q

● Detection of Cryptococcus spp. in CSF
● Diagnostic of meningitis when positive in CSF.

A

India ink stain

41
Q

Most widely used method of staining and observing fungi. Lactic acid preserves structures; slides can be made permanent.

A

Lactophenol cotton or aniline blue wet mount

42
Q

Clearing of specimen to make fungi more readily visible. Rapid detection of fungal elements.

A

Potassium hydroxide

43
Q

Examination of melanin pigment in fungal cell walls. Aids differentiation of melanin and hemosiderin pigments

A

Masson-Fontana stain

44
Q

Detection of fungi in histologic section. Best stain for detecting fungal elements

A

Methenamine silver stain

45
Q

Examination of secretions for malignant cells

A

Papanicolaou stain

46
Q

Stains fungal elements well; hyphae of molds and yeasts can be readily distinguished.

A

Periodic acid Schiff (PAS) stain

47
Q

Examination of fungal elements. Quickly performed and cost-effective.

A

Saline wet mount

48
Q

Examination of bone marrow or peripheral blood smears. Detects Histoplasma capsulatum and Cryptococcus spp

A

Wright’s stain

MYCOLOGY AND VIROLOGY (10 decks)

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