IDENTIFICATION OF SEVERAL FUNGAL CULTURE MEDIA Flashcards
Brain-heart infusion agar composition. Primary recovery of saprobic and pathogenic fungi
1.Brain-heart infusion
2.enzymatic digest of animal tissue,
3.enzymatic digest of casein,
4.dextrose
5. sodium chloride
Brain-heart infusion agar with antibiotics composition. Primary recovery of pathogenic fungi exclusive of dermatophytes
1.Brain-heart infusion
2.enzymatic digest of animal tissue,
3.enzymatic digest of casein,
4.dextrose,
5.sodium chloride,
6.antibiotics
Brain-heart infusion biphasic blood culture
bottles. Recovery of fungi from blood
1.Brain-heart infusion
2.peptone
3.glucose
4.disodium phosphate
Isolation and presumptive
identification of yeast and filamentous fungi
Chromogenic agar
Chromogenic agar composition:
Chromopeptone
Glucose
Chromogen mix
Chloramphenicol
Primary recovery of dermatophytes;
recommended as screening medium
Dermatophyte test medium
Dermatophyte test medium composition:
1.Dextrose
2.cycloheximide,
3.gentamycin
4.chloramphenicol
5.phenol red
Inhibitory mold agar composition. Primary recovery of pathogenic fungi exclusive of
dermatophytes
Chloramphenicol,
casein,
dextrose,
starch,
sodium phosphate,
magnesium sulfate,
sodium chloride,
manganese sulfate
Potato flake agar composition. Primary recovery of saprobic and pathogenic
fungi
Potato flakes
glucose,
cycloheximide,
chloramphenicol,
bromthymol blue
Mycosel agar COMPOSITION Primary recovery of dermatophytes
Cycloheximide,
chloramphenicol,
dextrose
Sabouraud Dextrose with Brain Heart
Infusion (SABHI) agar. Primary recovery of
saprobic and pathogenic fungi
Sabouraud dextrose
brain-heart infusion agar
Yeast-extract phosphate agar. Primary recovery of pathogenic fungi
exclusive of dermatophytes
Yeast extract,
dipotassium phosphate,
chloramphenicol
DIFFERENTIAL TEST MEDIA
Identification of Cryptococcus, Trichosporon, and Rhodotorula spp.
Christensen’s urea agar
Christensen’s urea agar composition
2% urea, phenol red
Identification of Candida albicans by
chlamydospore production Identification of C.
albicans by microscopic morphology
Cornmeal agar with Tween 80 and trypan
blue
Cornmeal agar with Tween 80 and trypan
blue composition
Cornmeal, Tween 80, trypan blue
Conversion of the dimorphic fungus
Blastomyces spp. from mold to yeast form
Cottonseed conversion agar
Cottonseed conversion agar composition
Cottonseed meal, glucose
Differential identification of Aspergillus spp.
Czapek’s agar
Czapek’s agar composition:
Sodium nitrate
sucrose
yeast extract
Identification of Cryptococcus
neoformans and Cryptococcus gattii
Niger seed agar (birdseed agar)
Niger seed agar (birdseed agar) composition:
Guizotia abyssinica seed,
dextrose,
chloramphenicol
Detection of nitrate reduction to confirm
Cryptococcus spp.
Nitrate reduction
medium
Nitrate reduction medium composition:
Potassium nitrate
peptone
meal extract
sulfanilic acid,
N-dimethyl-1-naphthylamine
Demonstration of pigment production by
Trichophyton rubrum Preparation of microslide cultures and sporulation
of dermatophytes
Potato dextrose agar
Potato dextrose agar composition
Potato infusion, D(+) glucose
Identification of Microsporum audouinii
Rice medium
Rice medium composition:
White rice extract
polysorbate 80
Identification of Trichophyton spp.
Trichophyton agars 1-7
Trichophyton agars 1-7 composition:
Casamino acids
dextrose
monopotassium phosphate,
magnesium sulfate
amino acids,
ammonium nitrate
Casamino acids, dextrose,
monopotassium phosphate,
magnesium sulfate, amino acids,
ammonium nitrate
Trichophyton agars 1-7
Urea agar composition:
Peptone,
dextrose,
sodium chloride,
monopotassium phosphate,
urea,
phenol red
Identification of yeasts by determining
fermentation
Yeast fermentation
broth
Yeast fermentation broth composition
Yeast extract,
peptone,
bromcresol purple
specific carbohydrate
Identification of yeasts by determining
carbohydrate assimilation
Yeast nitrogen-base agar
Yeast nitrogen-base agar composition
Ammonium sulfate
carbon source
This important procedure often can provide the first microbiologic proof of the cause of disease in patients with fungal infection and guide the selection of appropriate media to
support growth.
Direct Microscopic Examination
Detection of Mycobacteria, Nocardia spp. and some isolates of Blastomyces spp.
Acid-fast stain and partial acid-fast stain
● Detection of Mycobacteria and Nocardia
● Excellent screening tool; sensitive and affordable.
Auramine-rhodamine stain
Detects fungi rapidly because of bright fluorescence.
Calcofluor white stain
Commonly performed on most clinical specimens submitted for bacteriology; detects most fungi.
Gram stain
● Detection of Cryptococcus spp. in CSF
● Diagnostic of meningitis when positive in CSF.
India ink stain
Most widely used method of staining and observing fungi. Lactic acid preserves structures; slides can be made permanent.
Lactophenol cotton or aniline blue wet mount
Clearing of specimen to make fungi more readily visible. Rapid detection of fungal elements.
Potassium hydroxide
Examination of melanin pigment in fungal cell walls. Aids differentiation of melanin and hemosiderin pigments
Masson-Fontana stain
Detection of fungi in histologic section. Best stain for detecting fungal elements
Methenamine silver stain
Examination of secretions for malignant cells
Papanicolaou stain
Stains fungal elements well; hyphae of molds and yeasts can be readily distinguished.
Periodic acid Schiff (PAS) stain
Examination of fungal elements. Quickly performed and cost-effective.
Saline wet mount
Examination of bone marrow or peripheral blood smears. Detects Histoplasma capsulatum and Cryptococcus spp
Wright’s stain
