IDENTIFICATION OF SEVERAL FUNGAL CULTURE MEDIA Flashcards by Khienja Maverick Gonzaga

Brain-heart infusion agar composition. Primary recovery of saprobic and pathogenic fungi

1.Brain-heart infusion
2.enzymatic digest of animal tissue,
3.enzymatic digest of casein,
4.dextrose
5. sodium chloride

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Brain-heart infusion agar with antibiotics composition. Primary recovery of pathogenic fungi exclusive of dermatophytes

1.Brain-heart infusion
2.enzymatic digest of animal tissue,
3.enzymatic digest of casein,
4.dextrose,
5.sodium chloride,
6.antibiotics

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Brain-heart infusion biphasic blood culture
bottles. Recovery of fungi from blood

1.Brain-heart infusion
2.peptone
3.glucose
4.disodium phosphate

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Isolation and presumptive
identification of yeast and filamentous fungi

Chromogenic agar

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Chromogenic agar composition:

Chromopeptone
Glucose
Chromogen mix
Chloramphenicol

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Primary recovery of dermatophytes;
recommended as screening medium

Dermatophyte test medium

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Dermatophyte test medium composition:

1.Dextrose
2.cycloheximide,
3.gentamycin
4.chloramphenicol
5.phenol red

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Inhibitory mold agar composition. Primary recovery of pathogenic fungi exclusive of
dermatophytes

Chloramphenicol,
casein,
dextrose,
starch,
sodium phosphate,
magnesium sulfate,
sodium chloride,
manganese sulfate

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Potato flake agar composition. Primary recovery of saprobic and pathogenic
fungi

Potato flakes
glucose,
cycloheximide,
chloramphenicol,
bromthymol blue

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Mycosel agar COMPOSITION Primary recovery of dermatophytes

Cycloheximide,
chloramphenicol,
dextrose

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Sabouraud Dextrose with Brain Heart
Infusion (SABHI) agar. Primary recovery of
saprobic and pathogenic fungi

Sabouraud dextrose
brain-heart infusion agar

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Yeast-extract phosphate agar. Primary recovery of pathogenic fungi
exclusive of dermatophytes

Yeast extract,
dipotassium phosphate,
chloramphenicol

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DIFFERENTIAL TEST MEDIA
Identification of Cryptococcus, Trichosporon, and Rhodotorula spp.

Christensen’s urea agar

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Christensen’s urea agar composition

2% urea, phenol red

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Identification of Candida albicans by
chlamydospore production Identification of C.
albicans by microscopic morphology

Cornmeal agar with Tween 80 and trypan
blue

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Cornmeal agar with Tween 80 and trypan
blue composition

Cornmeal, Tween 80, trypan blue

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Conversion of the dimorphic fungus
Blastomyces spp. from mold to yeast form

Cottonseed conversion agar

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Cottonseed conversion agar composition

Cottonseed meal, glucose

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Differential identification of Aspergillus spp.

Czapek’s agar

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Czapek’s agar composition:

Sodium nitrate
sucrose
yeast extract

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Identification of Cryptococcus
neoformans and Cryptococcus gattii

Niger seed agar (birdseed agar)

Niger seed agar (birdseed agar) composition:

Guizotia abyssinica seed,
dextrose,
chloramphenicol

Detection of nitrate reduction to confirm
Cryptococcus spp.

Nitrate reduction
medium

Nitrate reduction medium composition:

Potassium nitrate
peptone
meal extract
sulfanilic acid,
N-dimethyl-1-naphthylamine

Demonstration of pigment production by Trichophyton rubrum Preparation of microslide cultures and sporulation of dermatophytes

Potato dextrose agar

Potato dextrose agar composition

Potato infusion, D(+) glucose

Identification of Microsporum audouinii

Rice medium

Rice medium composition:

White rice extract polysorbate 80

Identification of Trichophyton spp.

Trichophyton agars 1-7

Trichophyton agars 1-7 composition:

Casamino acids dextrose monopotassium phosphate, magnesium sulfate amino acids, ammonium nitrate

Casamino acids, dextrose, monopotassium phosphate, magnesium sulfate, amino acids, ammonium nitrate

Trichophyton agars 1-7

Urea agar composition:

Peptone, dextrose, sodium chloride, monopotassium phosphate, urea, phenol red

Identification of yeasts by determining fermentation

Yeast fermentation broth

Yeast fermentation broth composition

Yeast extract, peptone, bromcresol purple specific carbohydrate

Identification of yeasts by determining carbohydrate assimilation

Yeast nitrogen-base agar

Yeast nitrogen-base agar composition

Ammonium sulfate carbon source

This important procedure often can provide the first microbiologic proof of the cause of disease in patients with fungal infection and guide the selection of appropriate media to support growth.

Direct Microscopic Examination

Detection of Mycobacteria, Nocardia spp. and some isolates of Blastomyces spp.

Acid-fast stain and partial acid-fast stain

● Detection of Mycobacteria and Nocardia ● Excellent screening tool; sensitive and affordable.

Auramine-rhodamine stain

Detects fungi rapidly because of bright fluorescence.

Calcofluor white stain

Commonly performed on most clinical specimens submitted for bacteriology; detects most fungi.

Gram stain

● Detection of Cryptococcus spp. in CSF ● Diagnostic of meningitis when positive in CSF.

India ink stain

Most widely used method of staining and observing fungi. Lactic acid preserves structures; slides can be made permanent.

Lactophenol cotton or aniline blue wet mount

Clearing of specimen to make fungi more readily visible. Rapid detection of fungal elements.

Potassium hydroxide

Examination of melanin pigment in fungal cell walls. Aids differentiation of melanin and hemosiderin pigments

Masson-Fontana stain

Detection of fungi in histologic section. Best stain for detecting fungal elements

Methenamine silver stain

Examination of secretions for malignant cells

Papanicolaou stain

Stains fungal elements well; hyphae of molds and yeasts can be readily distinguished.

Periodic acid Schiff (PAS) stain

Examination of fungal elements. Quickly performed and cost-effective.

Saline wet mount

Examination of bone marrow or peripheral blood smears. Detects Histoplasma capsulatum and Cryptococcus spp

Wright’s stain