Cutaneous and Subcutaneous Mycoses PART1 Flashcards
Cutaneous Mycoses:
Microsporum
Trichophyton
Epidermophyton
Subcutaneous Mycoses
Mycetoma
Chromoblastomycosis
Phaeohyphomycosis
Sporotrichosis
-Most common fungal infections of humans. Usually referred to as tinea (Latin for “worm” or “ringworm”)
Cutaneous Mycoses/ TINEA
Ringworm of the body
Tinea corporis
Ringworm of the groin or “jock itch”
Tinea cruris
Ringworm of the scalp and hair
Tinea capitis
Ringworm of the beard
Tinea barbae
Ringworm of the nail
Tinea unguium
-Members of this genus are widely
distributed and are the most
important and common causes of
infections of the feet and nails
- Commonly seen in adult infections
- Most cosmopolitan species are
anthropophilic or “human loving”
- Hairs infected with organisms do not fluorescence under the UV light of a Woods lamp
Trichophyton
Calcofluor white/KOH preparations reveal the presence of hyaline septate hyphae or arthroconidia
Trichophyton
-Infected hairs may reveal the hair shaft to be filled with masses of large arthroconidia (4-7 um) in chains (endothrix infection). Infected hairs that show external masses of spores that
ensheaths the hair shaft (ectothrix infection)
Trichophyton spp.
infected hairs reveal hyphae and air spaces within the shaft
Trichophyton Shoenleinii
Trichophyton spp. Initial growth must be subcultured onto _____ to induce sporulation
cornmeal agar or potato dextrose agar
are the most common species recovered in the clinical laboratory
T. rubrum and T. mentagrophytes
is slow-growing, produces a flat/heaped-up
colony, generally WHITE TO REDDISH, with cottony or velvety surface
Trichophyton rubrum
Characteristic cherry-red color best observed on the reverse side of the colony (after 3-4 weeks of incubation)
T. rubrum
T. rubrum Two types of colonies may be produced:
fluffy and granular
microconidia are uncommon in most of the
fluffy strains and more common in the granular strains. Microconidia occur as small, teardrop-shaped conidia often borne laterally along the sides of the hyphae
- Macroconidia are less common; they appear in granular strains as thin-walled, smooth-walled, multi celled, cigar-shaped conidia with three to eight septa
- has no specific nutritional requirements
- Does not perforate hair in vitro or produce
urease
T. rubrum
produce two distinct colonial forms:
downy variety recovered from patients with tinea pedis and the granular variety recovered from lesions acquired by contact with animals
T. mentagrophytes
- Rapidly growing colonies
- Appear white to cream-colored or yellow
- Cottony or downy
- Coarsely granular to powdery
- May produce a few spherical microconidia
- Reverse side of colony is usually ROSE BROWN, occasionally orange to deep red (may be confused for T. rubrum)
T. mentagrophytes
-colonies sporulate freely;
- microconidia in grapelike clusters and thin-walled, smooth walled, cigar-shaped macroconidia measuring 6 × 20 μm to 8 × 50 μm, with two to five septa
- Macroconidia characteristically exhibit a definite narrow attachment to their base.
- Spiral hyphae may be found in one third of the isolates recovered.
T. mentagrophytes
- produce urease within 2 to 3 days after
inoculation onto Christensen’s urea agar - Perforate hair unlike T. rubrum
T. mentagrophytes
Responsible for an epidemic form of tinea capitis that commonly occurs in children
T. tonsurans
causes a low-grade superficial lesion of varying
severity and produces circular, scaly patches of
alopecia (loss of hair)
T. tonsurans