ICS - Microbiology Flashcards
What is meant by gram positive bacteria?
They have a thick peptidoglycan layer and no outer lipid membrane. They retain the colour of the crystal violet stain in the gram stain
What is meant by gram negative bacteria?
They have a thin peptidoglycan layer and have an outer lipid membrane
What does a gram stain tell you?
If you have a bacterial infection - will either show gram positive or gram negative
Approach to identifying bacteria - 3
- Gram +’ve / -‘ve
- Morphology/ shape - cocci, bacilli, etc.
- In clusters or chains
Colour of gram stain positive ?
purple
Colour of gram stain negative ?
pink
How many membranes do gram stain negative bacteria have?
2
Bacteria shapes
Cocci, bacilli, spirochaete
Coccus bacteria shape
Oval/ spherical
Bacilli bacteria shape
Rod shaped
Staphylcocci bacteria
gram-positive cocci that grow in clusters
Strepococci bacteria
gram-positive cocci that grow in pairs or chains
Define pathogen
disease causing microorganism
Define commensal
organism which colonises the host but does not cause disease
Define opportunist pathogen
Microbe that only causes disease if the host defenses are compromised
Define virulence/ pathogenicity
degree to which an organism is pathogenic
Define asymptomatic carriage
When a pathogen is carried harmlessly at a tissue site where it causes no disease
Purpose of the catalase test?
to differentiate between staphylococci and streptococci
Explaining the results of the catalase test
Enzyme in bacteria which catalyses the decomposition of H2O2 –> H2O and O2. Therefore, when a bacterium possesses catalase, when H2O2 is added, oxygen bubbles are observed. particular types of bacteria (e.g. Staphylococci) are catalase-positive
Catalase test
Add H2O2 to bacteria and see for bubbles (oxygen) = positive result
Results of catalase test?
Bubbling = staph
No bubbling = non staph
Coagulase enzyme
produced by Staphylococcus. aureus that converts soluble fibrinogen –> insoluble fibrin
What does the coagulase test differentiate between?
Staphylococci. aureus and other Staphylococci
Results of the coagulase test?
Staphylococcus. aureus - coagulase positive - clumping
Other Staphylococci - coagulase negative - no clumping
Gram stain test procedure
- Apply primary stain such as crystal violet to bacteria
- Add iodide which binds to crystal violet and fixes it to the cell wall
- Decolourise with ethanol/ acetone
- Counterstain with safranin (pink)
Haemolysis is the ability of bacteria to
Break down RBC in blood agar, it requires the expression of haemolysin
Substance in bacteria that causes haemolysis
Haemolysin
Beta haemolysis
Clear colourless zone appears around the bacteria colonies
e.g. Staphylococci pyogenes, Staphylococci agalactiae, etc.
Alpha haemolysis
Indistinct zone of partial lysis of RBCs around the colonies of bacteria, often gives a green/brown discolourisation
Important bacteria that you need to distinguish that is alpha haemolytic
streptococcus pneumoniae - causes lobar pneumonia and meningitis
How to test for streptococcus pneumoniae?
Perform the optochin test
Result of optochin test?
streptococcus pneumoniae are optochin +’ve, whereas other alpha streptococci are resistant
alpha streptococci that cause infective endocarditis?
streptococci viridans
result of optochin test for streptococci viridans?
resistant, no clear ring
Positive result of optochin test?
clear demarcated ring around optochin disc
Optochin
Antibiotic that will inhibit the growth of sensitive bacteria e.g. pneumococci
Staphylococcus aureus is beta haemolytic, therefore what two tests can identify this strain?
- Positive coagulase test (clumping)
- Positive beta haemolysis test - clear ring around colony
Gamma haemolysis
Implies no haemolysis
Bacteria sensitive to optochin
streptococci pneumoniae
Bacteria resistant to optochin
viridans streptococci and other alpha haemolytic streptococci
Oxidase test purpose?
Test if the bacteria contain cytochrome oxidase enzymes (in oxidative phosphorylation) - i.e. respires aerobically
All bacteria that are oxidase positive are –>
aerobic
bacteria that are oxidase negative are –>
either aerobic or anaerobic
Oxidase positive
blue result - bacteria is aerobic
Oxidase negative
no colour change
Macconkey agar only grows
gram-negative bacilli
Staphylococcus are gram
positive
Lancefield grouping
Method of grouping catalyse negative, coagulase negative bacteria based on bacterial carbohydrate surface antigens
Staphylococcus
- > 40 species
- Coagulase +’ve or -‘ve
- Catalase positive
- S. aureus - coagulase +’ve
- Normal habitat - nose or skin
Staphylococcus aureus - common clinical presentation
pain in shoulder, elevated temperature, causes osteomyelitis (bone infectio)
treat with flucloacillin for 3 months
Staphlococcus aureus
- Coagulase positive
- Spread by aerosol and touch
MRSA =
methicillin resistant Staphylococcus aureus
MRSA is resistant to
Beta-lactams antibiotics, gentamicin, erythromycin, tetracycline
Virulence factors of staphylococcus aureus
- Pore-forming toxins
- Proteases
- Toxic shock syndrome
Associated conditions with staphylococcus aureus
Wound infections, osteomyelitis, toxic shock syndrome, food poisoning
Two examples of coagulase negative staphylococci?
staphylococcus epidermis, staphylococcus saprophyticus
Three ways of classifying streptococci?
- haemolysis
- Lancefield typing
- Biochemical properties
Lancefield typing of both catalase and coagulase negative bacteria based on carbohydrate cell surface antigens - important groupings
Group. A - S. pyogenes
Group B - S agalactiae
Infections caused by Streptococci pyogenes
Cellulitis, tonsilitis, scarlet fever
Streptococci pneumoniae - common presentation
heavy smoker with nasal congestion and fever, gets cough and chest pain, rust coloured sputum, chest x-ray shows fluid in lung
Viridans streptococci is the collective name for
oral streptococci
Where is the normal commensal of Streptococci pneumoniae?
oropharynx
Infections caused by Streptococci pneumoniae?
Pneumonia, meningitis, otitis media (middle ear infection), sinusitis
Virulence/pathogenic factors of Streptococci pneumoniae?
- Polysaccharide capsule
- Cytotoxin
Types of bacterial toxins?
Endo and exotoxins
Endotoxin
Component of the outer membrane of bacteria - e.g. lipopolysaccharide in gram -‘ve bacteria
Exotoxin
secreted proteins of gram +’ve and gram -‘ve bacteria
Major difference betwen gram +’ve and gram -‘ve bacteria?
Presence of lipopolysaccharide on gram-negative bacteria
Lipopolysaccharides (LPOs)
Constituent of the outer membrane of gram-negative bacteria and are also referred to as endotoxins
Function of LPOs
Help protect bacterium from host defences and can contribute to illness in the host
Toxic portion of the LPO
Lipid A
Why do gram negative bacteria have more difficulty secreting toxins?
Needs to cross 2 membranes instead of one
4 major groups of gram-negative pathogens?
Proteobacteria, bacteroids, chlamydia, spirochaetes
Important gram +’ve bacilli
Listeria monocytogenes, propionibacterium acne (acne)
What does Macconkey agar indicate?
whether the bacteria can ferment lactose
Two key pathogenicity factors/ virulence factors of gram negative bacteria?
colonisation factors, toxins
colonisation factors of gram negative bacteria
adhesins and invasins
toxins released from gram negative bacteria?
secreted proteins
Enterobacteria (coliforms)
- Bacilli
- Most are motile due to flagella
- Macconkey agar can differentiate between lactose and non-lactose fermenting
Infections caused by E.COli
- wound infections
- UTIs
- Gastroenteritis
- Traveller’s diarrhoea