IC Vesicualr Traffic 1

Question 1

How is specificity in targeting achieved by the Vesicle and the target membrane?

Answer 1

Vesicles
- Surface Markers

Target Membrane
- COMPLEMENTARY Receptors

Question 2

Soluble Proteins need a _____ receptor which packages them into ______ vesicles

Answer 2

1. KDEL

2. COP1 coated

Question 3

What gives these vesicular compartments it’s molecular address?

Answer 3

Combination of Markers

Question 4

Cisternal Maturation Model

Answer 4

• Transport from the ER THROUGH the Center for the Golgi apparatus
• Via Matrix proteins
• Vesicles return via Periphery

Question 5

Two Hypothesis of transport of proteins W/In Golgi compartments

Answer 5

1. CIS-Ternal Maturation Model

2. Vesicle Transport Model

Question 6

Rab Protein Characteristics

Answer 6

• DIRECTS vesicle to Specific spots on the Target Membrane
• Plays role in specificity
• Activated by GTP hydrolysis

Question 7

Biosynthetic Pathway Characteristics

Answer 7

• Newly synthesized cargo
• Moved in FORWARD direction
• From ER to PM

Question 8

How are Proteins and other biomolecules transported in the cell?

Answer 8

Transport VESICLES

Question 9

Disassembly of the Clathrin Coat

Answer 9

Clathrin coat lost after Vesicle formation is COMPLETE

Question 10

ENDOCYTIC Pathway Characteristics

Answer 10

• Molecules coming from the OUTSIDE of the cell

- Usually ends up in the LYSOSOME

Question 11

Retrieval Pathway Characteristics

Answer 11

• Allows molec. to go BACK to its ORIGINAL location

- From PM to ER

Question 12

SNARE Proteins Characteristics

Answer 12

• Mediate FUSION of Vesicle to the Membrane
• Two types
  1. V-Snares
  2. T-Snares
• Interaction b/w V and T SNAREs allow them to WRAP around each other to form a BUNDLE

Question 13

Cis Golgi Network

Answer 13

• AKA CGN
• A network of fused vesicular Tubular clusters arriving FROM the ER
• Proteins and Lipids Enter Golgi via CGN

Question 14

What proteins play a role in Vesicle targeting?

Answer 14

• Rab Proteins

- SNARE Proteins

Question 15

Vesicle Transport Model

Answer 15

• Transport from the ER through the PERIPHERY for the Golgi apparatus
• Via Vesicles
• Vesicles return via Periphery

Question 16

V-Snares vs T-Snares

Answer 16

V

• Single polypeptide
• Found on VESICLE Membrane

T

• 2 to 3 proteins
• Found on TARGET membranes

Question 17

Vesicular Transport is _____.

Answer 17

Directional

Question 18

Assembly of the Clathrin Coat

Answer 18

• Introduces a Curve in the membrane
• ADAPTOR Proteins bind Clathrin AND Membrane-Bound Cargo Receptors
• Selective recruitment of membrane

Question 19

What is responsible for the PINCHING off of the vesicle? How is it Powered?

Answer 19

• DYNAMIN Helix
  And
• Associated Proteins
• Powered by GTP Hydrolysis

Question 20

How many types of Coated Vesicles are there?

Answer 20

3 Total

1. Clathrin
2. COP1
3. COP2

Question 21

Recruitment of Cargo into ER Transport Vesicles requires what?

Answer 21

• COP2
• Adaptor Proteins
• Exit ignal on Cargo RECEPTOR
• Exit Signal on Cargo PROTEIN

Question 22

The Faces of the Golgi Stack

Answer 22

2 Faces

• Cis: ENTRY
• Trans: EXIT

Question 23

How many vesicular Transport systems are there? Names?

Answer 23

3 Total

1. Biosynt.-SECRETORY
2. ENDOCYTIC
3. RETREIVAL

Question 24

Contents in a Vesicle are called what?

Answer 24

CARGO

Question 25

Role of NSF

Answer 25

• Works with accessory proteins

- Dissociate the V/T SNAREs from each other after fusion has occurred

Question 26

Clathrin Characteristics

Answer 26

Mediate Transport from:

• Golgi Apparatus

And

• Plasma Membrane

Question 27

Trans Golgi Network

Answer 27

• AKA TGN
• The region that allows exit of proteins and lipids to next step of secretory pathway
• Plays a role in Glycosylation