hw/quiz 8-10

Question 1

Synthesis of a repressible enzyme is stopped by the
Selected
Answers:
1) substrate binding to the repressor
2) corepressor binding to the operator
3) end product binding to the promoter.
4) corepressor-repressor complex binding to the operator.

Answer 1

Answers:
Correct
corepressor-repressor complex binding to the operator.

Question 2

Which of the following is NOT based on nucleic-acid hybridization?

Answers:

1) Southern blotting
2) FISH
3) PCR
4) Western blotting

Answer 2

Selected Answer:
Correct
Western blotting

Question 3

The initial effect of ionizing radiation on a cell is that it causes

Answers:

1) the formation of highly reactive ions.
2) DNA to break
3) bonding between adjacent thymines
4) base substitutions

Answer 3

Selected Answers:

the formation of highly reactive ions.

Question 4

A colleague has used computer modeling to design an improved enzyme. To produce this enzyme, the next step is to

Answers:

1) determine the nucleotide sequence for the improved enzyme.
2) determine the RNA sequence for the improved co-enzymes.
3) synthesize the gene for the improved enzyme

Answer 4

Selected Answer:
Correct
determine the nucleotide sequence for the improved enzyme.

Question 5

A biochemical test, in the microbiology world, is used to determine

1) staining characteristics.
2) amino acid sequences of enzymes
3) nucleic acid-base composition of DNA sequences.
4) capability of a microbe to perform a specific enzymatic activity.

Answer 5

Selected Answer:
Correct
capability of a microbe to perform a specific enzymatic activity.

Question 6

Genetic change in bacteria can be brought about by

1) mutation
2) conjugation
3) transduction
4) all of the answers are correct

Answer 6

all of the answers are correct

Question 7

Conjugation differs from reproduction because conjugation

1) replicates DNA
2) transfers DNA vertically, to new cells.
3) transcribes DNA to RNA
4) transfers DNA horizontally, to nearby cells without those cells undergoing replication

Answer 7

transfers DNA horizontally, to nearby cells without those cells undergoing replication

Question 8

An advantage of synthetic DNA over genomic or cDNA is the ability to

1) insert desired restriction sites into the DNA sequence.
2) make DNA from cellular RNA and the enzyme reverse transcriptase
3) obtain genes that lack introns
4) obtain genes that lack exons.

Answer 8

insert desired restriction sites into the DNA sequence.

Question 9

An enzyme that copies DNA to make a molecule of RNA is

1) RNA polymerase
2) DNA ligase
3) DNA helicase
4) transposase.

Answer 9

RNA poymerase

Question 10

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

1) gene guns
2) protoplast fusion
3) Ti plasmids and Agrobacterium
4) microinjection

Answer 10

Correct

microinjection

Question 11

A population of cells carrying a desired plasmid is called a

Answers:

1) clone
2) library
3) Southern blot
4) PCR

Answer 11

Correct

clone

Question 12

According to the operon model, for the synthesis of an inducible enzyme to occur, the

1) end-product must not be in excess.
2) substrate must bind to the enzyme
3) substrate must bind to the repressor
4) repressor must bind to the operator.

Answer 12

substrate must bind to the repressor

Question 13

What is the outstanding characteristic of the Kingdom Fungi
Answers:
1) What is the outstanding characteristic of the Kingdom Fungi
2) Members absorb dissolved organic matter.
3) Members absorb dissolved inorganic matter.
4) All members are microscopic

Answer 13

Members absorb dissolved inorganic matter.

Question 14

You have a small gene that you wish replicated by PCR. After 3 replication cycles, how many double-stranded DNA molecules do you have?

Answer 14

8

Question 15

Which of the following statements about the members of the Kingdom Animalia is FALSE
Answers:
1) They are multicellular
2) They are composed of eukaryotic cells.
3) They undergo photosynthesis
4) They ingest nutrients through a mouth.

Answer 15

They undergo photosynthesis

Question 16

An enzyme produced in response to the presence of a substrate is called a(n)

1) inducible enzyme
2) respressible enyzme
3) restriction enzyme
4) operator

Answer 16

inducible enzyme

Question 17

The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes

1) replica plating possible.
2) direct selection possible
3) the recombinant cell dangerous
4) the recombinant cell unable to survive.

Answer 17

direct selection possible

Question 18

Assume you have discovered a cell that produces a lipase that works in cold water for a laundry additive. You can increase the efficiency of this enzyme by changing one amino acid. This is done by

1) irradiating the cells
2) site-directed mutagenesis
3) enrichment
4) selective breeding.

Answer 18

site-directed mutagenesis

Question 19

Phage typing is based on the fact that

1) bacteria are destroyed by viruses.
2) viruses cause disease
3) bacterial viruses attack only members of a specific species
4) Staphylococcus causes infections

Answer 19

bacterial viruses attack only members of a specific species