Chp 8-10 chp review

Question 1

DNA was discovered in… by?

Answer 1

in 1869 by Fredrich Miescher

Question 2

how did fredriech miescher discover dna

Answer 2

By isolating the nuclei of white blood cells, he extracted an acidic molecule he called “Nuclein”.

Question 3

Three Methods of Gene Transfer in Bacteria

Answer 3

transformation
transduction
conjugation

Question 4

“naked” DNA is

Answer 4

is transferred from one bacterium to another in solution during transformation

Question 5

DNA fragments are released as what during transformation

Answer 5

double-stranded DNA into the medium during transformation

Question 6

Endonucleases cut what during transformation

Answer 6

double-stranded DNA in solution and the resulting fragments separate—only single-stranded molecules are transferred during transformation

Question 7

The transferred DNA are…. during transformation are

Answer 7

is spliced into the recipient cell’s DNA

Question 8

Transformation occurs naturally among

Answer 8

some bacteria

Question 9

It is used in the laboratory to create, as well as….

Answer 9

create recombinant DNA and is also used to study the effects of introducing DNA into a cell, and in mapping gene locations.

Question 10

DNA is transferred from one bacterium to another via a

Answer 10

bacteriophage and then incorporated into the recipient’s DNA during transduction

Question 11

During bacteriophage, which takes place during transduction what may happen to a bacterial virus

Answer 11

they may be virulent or temperate