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HTMTLE > HTMLE DAY 6 > Flashcards

HTMLE DAY 6 Flashcards

1
Q

what is the most commonly used fixative?

A

aldehyde fixative

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2
Q

most widely used aldehyde fixative

A

10% formaldehyde solution

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3
Q

ALDEHYDE FIXATIVE FOR 10% FORMOL SALINE

A

CNS TISSUE

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4
Q

ALDEHYDE FIXATIVEBEST FIXATIVE FOR TISSUE CONTAINING IRON PIGMENT

A

10% BNF

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5
Q

BEST GENERA; TISSUE FIXATIVE PH

A

7

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6
Q

ALDEHYDE FIXATIVE TAHT IS PRESERVE PLASMA PROTEIN BETTER FOR ELECTRON MICROSCOPY

A

GLUTARALDEHYDE

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7
Q

ALDEHYDE FIXATIVE USED FOR PRESERVATION OF LIPIDS

A

FORMOL-CALCIUM

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8
Q

MSOST COMMON METALLIC FIXATIVE MAY PRODUCE BLACK GRANULAR DEPOSITS ON TISSUE

A

MERCURIC CHLORIDE

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9
Q

METTALIC FIXATIVE FOR BONE MARROW

A

B-5 FIXATIVE

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10
Q

PRESERVE CHROMATIN TISSUE

A

CHROMATE

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11
Q

CHROMATE FIXATIVE USED FOR PRESERVATION OF CARBS AND PROTEIN

A

CHROMIC ACID

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12
Q

GENERALLY ACID MUCOPOLUSACCHARIDES IN WHARTON’S JELLY

A

LEAD FIXATVIE

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13
Q

WILL PRODUCE EXCESSIVE YELOW STAINING IN THE TISSUUE

A

PICRATE FIXATIVE

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14
Q

TRUE OR FALSE: PICRATE FIXATIVE MUST NEVER BE WASHED IN WATER BEFORE DEHYDRATION

A

TRUE

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15
Q

PICRIC FIXATIVE USED FOR FIXATION OF EMBRYOS CONTAING PICRIC ACID

A

BOUIN’S SOLUTION

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16
Q

FIXES NUCLEOPROTEIN BUT DESTROY MITOCHONDRIA AND GOLGI BODIES

A

GLACIAL ACETIC ACID

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17
Q

DISADVANTAGE OF THIS FIXATIVE: POLARIZATION- GLYCOPROTEIN GRANULES MOVE TOWARDS THE POLES R ENDS OF CELLS

A

ALCOHOL FIXATIVE

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18
Q

ALCOHOL FIXATIVEFOR BLOOD SMEAR AND BM TISSUE

A

METHANOL

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19
Q

ALCOHOL FIXATIVE FOR CYTOLOGY

A

ETHANOL

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20
Q

ALCOHOL FIXATIVE THAT IS MOST RAPID FIXATIVE FOR CHROMOSOMES

A

CARNOY’S FLUID

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21
Q

ALCOHOL FIXATIVE THAT IS USEFUL IN PRESERVING SPUTUM

A

GENDRE’S ALCOHOLIC FORMALIN

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22
Q

ALCOHOL FIXATIVE FOR BOTH NUCLEAR AND HISTOCHEMICAL FIXATIVE

A

NEWCOMER’S FLUUID

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23
Q

FOE ELECTRON MICROSCOPY THAT IS SHOULD NEVER KEPT IN A DARK COLORED, CHEMICALLY CLEAN BOTTLE TO PREVENT EVAPORATION

A

OSMIUM TETRAOXIDE FIXATIVE

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24
Q

OSMIUMM TETRAOXIDE FIXATVE THAT USED FOR NUCLEUS

A

FLEMMING’S SOL WITH GLACIAL ACETIC ACID

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25
OSMIUMM TETRAOXIDE FIXATVE THAT USED FOR CYTPLASMIC DETAILS
FLEMING’S SOLUTION WITHOUT GLACIAL ACETIC ACID
26
USED FOR DX OF RABIES
ACETONE
27
HEAT FIXATION USED FOR BACTERIAL SMEAR
DIRECT FLAMING FIXATION
28
OTHER STAIN FOR ELECTRON MICROSCOPY THAT ISE BEST STAIN FO EM
URANYL ACETATE
29
OSMIUM TETROXIDE FIXATIIVES PROCDUCES BLACK PRECIPITATE HOW COULD YOU PREVENT IT?
ADD SATURATED ACQEOUS HGCL2
30
WHAT IS THE REMEDY FOR THE BLACK PRECIPITATE PRODCED BY THE OSMIUM TETRAOXIDE
DISSOLVE IN COLD WATER
31
RECOMMENDED RATIO FOR FLUID TO TISSUE FOR DECLACIFICATION
20:1
32
RECOMMENDED RATIO FOR FLUID TO TISSUE FOR DECLACIFICATION USING OSMIUM TETRAOXIDE
5-10:1
33
TRUE OR FALSE HEAT CAN HASTEN THE DELCACIFICATION BUT IT ALSO INCREASES THE DAMAGING EFFECT ON TISSUES
TRUE
34
AT WHAT TEMP DOES IMPAIRED NUCLEAR STAINING FOR VAN GIENSON STAIN FOR COLLAGEN FIBERS
AT 37C
35
AT WHAT TEMP DOES THE TISSUE WILL UNDERGO COMPLETE DIGESTION WITHIN 24-48 HRS
AT 55C
36
OPTIMUM TEMP FORR DECALCIFICATION
ROOM TEMP 18C-30
37
IDEAL TIME REQUIRED FOR DECALCIFICATION OF TISSUE ?
RO24-48 HRS
38
DENSE BONE TISSUE USUALLY REQUIRE UP TO ___ OR LONGER TO COMPLETE THE PROCESS OF DECALCIFICATION
14 DAYS
39
WHAT ARE THE 4 DECALCIFYING AGENT
ACIDS CHELATING AGENT ION EXHANGE RENSIN ELECTRONIC IONIZATION
40
MOST COMMON CHELATING AGENT
EDTA
41
MOST COMMON ACID DECALCIFYING AGENT
NITRIC ACID
42
MOST RAPID DECALCIFYING AGENT
PHLOROGLUNIC NITRIC ACID
43
TYPE OF NITRIC ACID THAT IS BOTH FIXATIVE AND DECALCIFYING AGENT
FORMIC ACID
44
CONSIDERED TO BE THE BEST GENERAL DECLACIFYING AGENT
5% FORMIC ACID
45
RECOMMENDED FOR SMALL PIECES OF BONES AND TEETH
FORMIC ACID
46
TYPE OF ACID DECALCIFYING AGENT THAT IS RECOMMENDED FOR TEETH AND SMALL PICEDS OF BONE
VON EBNER’S FLUID
47
3 WAYS TO MEASURE EXTENT OF DECALCIFICATION
PHYSICAL. MECAHNICAL XRAY OR RADIOLOGIC METHOD CHEMICAL METHOD
48
USED FOR UNDULY HARD TISSUE THAT MAY DAAGE THE MICROTOME KNOVES
TISSUE SOFTNER
49
WHAT ARE THE EXAMPLE OF TISSUE SOFTENER
4% AQ PHENOL MOLLIFLEX 2% HCL 1% HCL IN 70% ALCOHOL PERENYI’S FLUID
50
REOVED THE DECAL AGENT USING LITHIU CARBONATE WASH WITH RUNNING WATER
POST DECAL
51
HOW MANY MINS OR HOURS IIT TAKES FOR SMALL BONE TO POST DECAL?
30 MINS
52
HOW MANY HOURS OR MINS DOES THE POST DECAL FOR LARGE BONE WIL TAKE UP?
1-4 HRS
53
AIM TO REMOVED THE FIXATIVE AND WATER FROM THE TISSUE AND REPLACING THEM WITH DEHYDRATING FLUID IN PREPARATION FOR IMPREGNATION
DEHYDRATION
54
ALL TEH ACQUEOUS TISSUE FLUID ARE REMOVED BUT WITH LITTLE DISRUPTION TO THE TISSUE DUE TO DIFFUSION CURRENTS
INCREASING STRENGTHS
55
DEHYDRATION AGENT USED FOR DELICATE SPX
30% ETOH
56
ASCENDING GRADE OF ALCHOL IN DEHYDRATION
65-70-75-85-95 ABSOLUTE ALCOHOL
57
COMMONLY USED DEHYDRATING AGENTS
ALCOHOL ACETONE DIOXANE CELLOSOLVE THF TRIETHYL PHOSPHATE
58
DEHYDRATING AGENT BOTH FIXATIVE AND DEHYDRATING AGENT
ACETONE
59
DEHYDRATION AGENT BOTH DEHYDRATING AND CLEARING AGENT
DIOXANE
60
BOTH DEHYDRATING AND CLEARING AGENT
THF
61
ADDITIVES USED FOR DEHYDRATING AGENTS
4% PHENOL +N 95% ETOH BATH ANHYDROUS COPPER SULFATE ALCOHOL
62
TYPE OF ALCOHOL THAT IS USED FOR ROUTINE DEHYDRATIOON OF TISSUE
ETHANOL
63
TYPE OF ALCHOL THAT IS “BEST DECALCIFYING AGENT”
ETHANOL
64
TYPE OF ALCOHOL THAT IS USED FOR BLOOD AND TISSUE FILMS
METHANOL
65
TYPE OF ALCOHOL THAT IS USED FOR ANIMAL ANG PLANTS MICRO TECHNIQUES
BUTYL ALCOHOL
66
TYPE OF ALCOHOL THAT IS USED FOR MICROWAVE PROCESSING
ISOPROPHYL ALCOHOL
67
INDICATION OF COMPLETE DEHYDRATION
ABH CUSO4 WHITE BLUE IF PRESENT OF WATER XYLENE TURNED MILKY
68
WHAT IS THE GENERAL RULE IN DEHYDRATION
AMOUNT ON EACH STAGE SHOULD NOT BE LESS THAN 10X THE VOLUME OF THE TISSUE

HTMTLE (7 decks)

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