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HTMTLE > HISTOPATH DAY 5 > Flashcards

HISTOPATH DAY 5 Flashcards

1
Q

2 METHODS OF PREPARING FROZEN SECTION

A

COLD KNIFE PROCEDURE
CRYOSTAT PROCEDURE

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2
Q

WHAT MICROTOME IS USED FOR COLD KNIFE PROCEDURE

A

FREEZING MICROTOME

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3
Q

TISSUE BLOCK FOR COLD KNIFE PROCEDURE

A

3-5UM

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4
Q

THE POINT AT WHICH SECTIONS MAY BE CUT 10UM

A

DEW LINES

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5
Q

OPTIMUM CONDITION FOR SECTIONING KINFE

A

-40 C TO -60C

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6
Q

OPTIMUM CONDITION FOR SECTIONING TISSUE

A

-5 TO -10

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7
Q

OPTIMUM CONDITION FOR SECTIONING ENVIRONMENT

A

0 TO -10

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8
Q

A REFRIGERATED CABINET IN WHICH A MODIFIED MICROTOME IS HOUSE

A

CRYOSTAT

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9
Q

WHAT MICROTOME I USED IN CRYOSTAT PROCEDUERE

A

ROTARY MICROTOME

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10
Q

WHAT IS THE REASON WHY THERE IS AN ICE CRYSTAL OR DISTORTION OF TISSUE

A

SLOW FREEZING

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11
Q

METHODS OF FREEZING

A

LIQUID NITROGEN
ISOPENTANE
AEROSOL SPRAY
CARBON DIOXIDE

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12
Q

METHOD OF FREEZING THAT IS MOST RAPID ADN COMMONLY AVAILABLE

A

LIQUID NITROGEN

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13
Q

TEMP FOR LIQUID NITROGEN

A

-190 C

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14
Q

METHOD OF FREEZING THAT IS MADE OF FLUORINATED HYDRICARBONS AND NOT FOR MUSCLE

A

AEROSOL SPRAY

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15
Q

THIS METHOD OF FREEZING BECAME LIQUID AT RT

A

ISOPENTANE

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16
Q

WHAT IS THE DEGREE CELSIUS OF CARBON DIOXIDE IN METHODS OF FREEZING

A

-50C

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17
Q

USED SYNTHETIC WATER-SOLUBLE GLYCOLS FOR CRYOSTAT SECTION

A

MOUTING OF TISSUE BLOCK

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18
Q

WHAT IS THE TEMP OF PLASTIC DISOENSER FOR BRAIN, LN , LIVER SOLEEN, UTERINE, CURRETING SOFT CELLULAR TUMORS

A

-5 TO -15 C

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19
Q

FOR NON FATTY, BREAST TISSUE , OVARY PROSTATE, TONGUE AND GIT

A

-15 TO -25

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20
Q

FOR FATTY BREAST AND ORNENTAL TISSUE

A

-35C

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21
Q

INVOLVES DIFFERENT PROCEDURES THAT HAVE BEEN ADOPTED FOR THE PREPARATION OF MATERIALS AND TISSUE FOR MICROSCOPIC EXAMINATION

A

HISTOPATHOLOGIC TECHNIQUES

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22
Q

ENNUMERATE THE TISSUE PROCESSING (IN ORDER)

A

NUMBERING
FIXATION
DEHYDRATION
CLEARING
WAX IMPREGNATION
BLOCKING
TRIMMING
SECTIONING
STAINING
MOUNTING
LABELING

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23
Q

1STY AND MOST CRITICAL STEP IN HISTOPATH TECHNIQUES

A

FIXATION

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24
Q

WHAT IS THE PRIMARY AIM OF FIXATION

A

PRESERCE THE MORPHOLOGY AND CHEM INTEGRITY OF TISSUE

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25
SECONDARY AIM OF FIXATION
SLIGHTLY HARDEN THE TISSUE TO PROTECT IT FROM FURTHER HANDLING SPX
26
CLASSICALLY DEFINED AS THE KILLING, PENETRATION AND HARDENING OF TISSUE
FIXATION
27
WHAT ARE THE FACTORS THAT INVOLVED IN FIXATION
HYDROGEN ION CONCENTRATION TEMPERATURE THICKNESS OF SECTION OSMOLALITY CONCENTARTION PENETRATION VOLUME EXPOSURE
28
WHAT IS THE IDEAL PH FOR TISSUE
PH 6-8
29
TEMPERATURE AT RT
15-25 C
30
WHAT IS THE REQUIREMENT TEMP FOR ELECTRONMICROSCOPY
0-4C
31
THICKNESS OF SECTION FOR ELECTRON MICROSCOPY
1-2MM
32
THICKNESS OF SECTION FOR LIGHT MICROSCOPY
2CM2
33
WHAT IS THE RIGHT OSMOLALITY OF FIXATION?
340 MOSM ISOTONIC 400-450 SLIGHTLY HYPERTONIC
34
WHAT IS THE CONCENTRATION OF FIXATIVE
10% FORMALIN 3% GLUTARALDEHYDE 0.25 GLUTARALDEHYDE FOR ELECTRON MICROSCOPY
35
HOW MANY MM PER HOUR PENETRATION OF FIXATION TO THE TISSUE
1 MM PER HOUR
36
WHAT IS THE VOLUME OF TISSUE TO FIXATIVE
1: 20 -25
37
HOW MANY HOURS DOES TISSUE NEED TO EB EXPOSED IN FIXATIVE
24-48 HOURS
38
WHAT WILL HAPPEN TO THE TISSUE IF IT IS EXPOSED TO THE FIXATIVE FOR MORE THAN 48 HOURS
TISSUE WILL HARDENED
39
FACTORS AFFECTS THE FIXATION RETARTED BY:
SIZE AND THICKNESS OF TISSUE BLOOD MUCUS FATS COLD TEMPERATURE
40
FACTORS THAT AFFECT FIXATION ENHANCED BY
SIZE AND THICKNESS OF THE TISSUE AGITATION MODERATE HEAT (37C-56C)
41
PERMITS THE GENERAL MICRSCOPIC STUDY OF TISSUE STRUCTURE WITHOUT ALTERING THE STRUCTURAL PATTERN AND NORMAL INTRACELLULAR RELATIONSHIP OF TISSUE
MICROANATOMIC FIXATIVE
42
3TYPES OF FIXATIVE ACCRODING TO ACTIONS
MICROANATOMIC FIXATIVE CYTOLOGIC FIXATIVE HISTOCHEMICAL FIXATIVE
43
EXAMPLE OF MICROANATOMIC FIXATIVE
10% FORMOLSALINE 10% NBF HEIDENHAIN'S SUSA FORMOL SUBLIMATE ZENKER'S FLUID BOUIN'S FLUID BRASIL SOLUTION
44
USED TO PRESERVE SPECIFIC PARTS/ELEMENTS OF TISSUE
CYTOLOGICAL FIXATIVE
45
2 TYPES OF CYTOLOGICAAL FIXATIVE
CYTOPLASMIC NUCLEAR
46
TYPE OF CYTOLOGICAL FIXATIVE THAT CONTAINS GLACIAL ACETIC ACID
NUCLEAR
47
TYPE OF CYTOLOGICAL FIXATIVE THAT DOES NOT CONTAINS GLACIAL ACETIC ACID
CYTOPLASMIC
48
EXAMPLE OF NUCLEAR CYTOLOGICAL FIXATIVE`
FLEMMING SOLUTION WITH GLACIAL ACETIC ACID CARNOY'S FLUID BOUIN SOLUTION NEWCOMER'S FLUID HEIDENHAIN SUSA
49
EXAMPLE OF CYTOPLASMIC CYTOLOGICAL FIXATIVE
FLEMING'S SOLUTION WITHOUT GLACIAL ACETIC ACID HELLY'S FLUID REGAUD'S OR MOLLER'S FLUID, ORTH'S FLUID
50
USED TO PRESERVE CHEMICAL CONSTITUENT OF THE CELL
HISTOCHEMICAL FIXATIVE
51
EXAMPLE OF HISTOCHEMICAL FIXATIVE
10% FORMOL SALINE ABSOLUTE ETHYL ALCOHOL ACETONE NEWCOMER'S FLUID
52
2 TYPES OF FIXATIVE ACCORDING TO COMPOSITION
SIMPLE FIXATIVE AND COMPOUND FIXATIVE
53
2 TYPES OF FIXATIVE ACCRODING TO MECHANISM OF ACTIONS
ADDITIVE FIXATIVE NON-ADDITIVE FIXATIVE
54
THEY CHEMICALLY ALTER THE TISSUE BY BONDING WITH IT AND ADDING THEMSELVES TO THE TISSE
ADDITIVE FIXATIVE
55
EXAMPLEOF ADDITIVE FIXATIVE
MERCURIC CHLORIDE FORMALDEHYDE CHROMIUM TRIOXIDE PICRIC ACID GLUTARALDEHYDE OSMIUM TETROXIDE ZINC SULFATE CHLORIDE
56
ACT ONA TISSUE WITHOUT CHEMICALLY COMBINING WITH IT
NON-ADDITIVE FIXATIVES
57
THEY ACT BY DISSOCIATING WATER FROM THE TISSUE PROETIN GROUPS CAUSING SHRINKAGE AND HARDENING OF THE TISSUE IF OVERFIXATION OCCURS
NON-ADDITIVE FIXATIVE
58
ACT BY CREATING NETFWORK THAT ALLOWS THE SOLUTION TO READILY PENETRATE THE INTERIOR OF THE TISSUE
COAGULANT FIXATIVE
59
CREATE A GEL THAT MAKES IT DIFFICULT TO PENETRATE BY SUBSEQUENT SOLUTIONS
NON-COAGULANT FIXATIVE
60
SELD DIGESTION OF THE CELL BY ITS OWN FERMENT
AUTOLYSIS
61
WHAT IS THE BEST REMEDY FOR AUTOLYSIS
PREVENTION
62
HOW TO PREVENT AUTOLYSIS
MINIMIZED COLD ISCHEMIA TIME ENSURE THE RATIO OF FIXATIVE AND TISSUE
63
WHAT TECHNIQUE SHOULD BE USED IN THE SURGICAL PATHOLOGY CUTTING ROOM FOR UTERUS SPX
BIVALVE TECHNIQUES
64
WHAT TECHNIQUE SHOULD BE USED IN THE SURGICAL PATHOLOGY CUTTING ROOM FOR GI TRACT
CUT THE WALL OF INTESTINE
65
WHAT TECHNIQUE SHOULD BE USED IN THE SURGICAL PATHOLOGY CUTTING ROOM FOR EYE
INJECT FORMOL ALCOHOL BEFORE FIXATIVE
66
WHAT TECHNIQUE SHOULD BE USED IN THE SURGICAL PATHOLOGY CUTTING ROOM FOR BRAIN
SUSPEND TO 10% BUFFERED FORMALIN FOR 3-4 WKS
67
WHAT TECHNIQUE SHOULD BE USED IN THE SURGICAL PATHOLOGY CUTTING ROOM FOR HARD TISSUE- LENDRUM'S METHOD
SPXX WASH IN RUNNING WATER OVERNIGHT ADN PLACE TO 4% PHENOL FOR 1-3 DAYS
68
EXAMPLE OF HARD TIISUE
CERVIX AND UTERUS
69
four principle of ethics
autonomous beneficence non-maleficence justice
70
5 bioethical issues
Euthanasia and assisted suicide Female genital mutilation Abortion Contraception organ donor
71
HB 564
Patient has the right the refuse diagnostic and medical treatment.
72
Patient has the right to terminate his/her life upon will or with his/her physician assistance
HB 8148

HTMTLE (7 decks)

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