Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

HTMTLE > HISTOPATH DAY 5 > Flashcards

HISTOPATH DAY 5 Flashcards

1
Q

2 METHODS OF PREPARING FROZEN SECTION

A

COLD KNIFE PROCEDURE
CRYOSTAT PROCEDURE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

WHAT MICROTOME IS USED FOR COLD KNIFE PROCEDURE

A

FREEZING MICROTOME

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

TISSUE BLOCK FOR COLD KNIFE PROCEDURE

A

3-5UM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

THE POINT AT WHICH SECTIONS MAY BE CUT 10UM

A

DEW LINES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

OPTIMUM CONDITION FOR SECTIONING KINFE

A

-40 C TO -60C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

OPTIMUM CONDITION FOR SECTIONING TISSUE

A

-5 TO -10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

OPTIMUM CONDITION FOR SECTIONING ENVIRONMENT

A

0 TO -10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

A REFRIGERATED CABINET IN WHICH A MODIFIED MICROTOME IS HOUSE

A

CRYOSTAT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

WHAT MICROTOME I USED IN CRYOSTAT PROCEDUERE

A

ROTARY MICROTOME

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

WHAT IS THE REASON WHY THERE IS AN ICE CRYSTAL OR DISTORTION OF TISSUE

A

SLOW FREEZING

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

METHODS OF FREEZING

A

LIQUID NITROGEN
ISOPENTANE
AEROSOL SPRAY
CARBON DIOXIDE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

METHOD OF FREEZING THAT IS MOST RAPID ADN COMMONLY AVAILABLE

A

LIQUID NITROGEN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

TEMP FOR LIQUID NITROGEN

A

-190 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

METHOD OF FREEZING THAT IS MADE OF FLUORINATED HYDRICARBONS AND NOT FOR MUSCLE

A

AEROSOL SPRAY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

THIS METHOD OF FREEZING BECAME LIQUID AT RT

A

ISOPENTANE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

WHAT IS THE DEGREE CELSIUS OF CARBON DIOXIDE IN METHODS OF FREEZING

A

-50C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

USED SYNTHETIC WATER-SOLUBLE GLYCOLS FOR CRYOSTAT SECTION

A

MOUTING OF TISSUE BLOCK

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

WHAT IS THE TEMP OF PLASTIC DISOENSER FOR BRAIN, LN , LIVER SOLEEN, UTERINE, CURRETING SOFT CELLULAR TUMORS

A

-5 TO -15 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

FOR NON FATTY, BREAST TISSUE , OVARY PROSTATE, TONGUE AND GIT

A

-15 TO -25

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

FOR FATTY BREAST AND ORNENTAL TISSUE

A

-35C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

INVOLVES DIFFERENT PROCEDURES THAT HAVE BEEN ADOPTED FOR THE PREPARATION OF MATERIALS AND TISSUE FOR MICROSCOPIC EXAMINATION

A

HISTOPATHOLOGIC TECHNIQUES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

ENNUMERATE THE TISSUE PROCESSING (IN ORDER)

A

NUMBERING
FIXATION
DEHYDRATION
CLEARING
WAX IMPREGNATION
BLOCKING
TRIMMING
SECTIONING
STAINING
MOUNTING
LABELING

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

1STY AND MOST CRITICAL STEP IN HISTOPATH TECHNIQUES

A

FIXATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

WHAT IS THE PRIMARY AIM OF FIXATION

A

PRESERCE THE MORPHOLOGY AND CHEM INTEGRITY OF TISSUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

SECONDARY AIM OF FIXATION

A

SLIGHTLY HARDEN THE TISSUE TO PROTECT IT FROM FURTHER HANDLING SPX

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

CLASSICALLY DEFINED AS THE KILLING, PENETRATION AND HARDENING OF TISSUE

A

FIXATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

WHAT ARE THE FACTORS THAT INVOLVED IN FIXATION

A

HYDROGEN ION CONCENTRATION
TEMPERATURE
THICKNESS OF SECTION
OSMOLALITY
CONCENTARTION
PENETRATION
VOLUME
EXPOSURE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

WHAT IS THE IDEAL PH FOR TISSUE

A

PH 6-8

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

TEMPERATURE AT RT

A

15-25 C

30
Q

WHAT IS THE REQUIREMENT TEMP FOR ELECTRONMICROSCOPY

A

0-4C

31
Q

THICKNESS OF SECTION FOR ELECTRON MICROSCOPY

A

1-2MM

32
Q

THICKNESS OF SECTION FOR LIGHT MICROSCOPY

A

2CM2

33
Q

WHAT IS THE RIGHT OSMOLALITY OF FIXATION?

A

340 MOSM ISOTONIC
400-450 SLIGHTLY HYPERTONIC

34
Q

WHAT IS THE CONCENTRATION OF FIXATIVE

A

10% FORMALIN
3% GLUTARALDEHYDE
0.25 GLUTARALDEHYDE FOR ELECTRON MICROSCOPY

35
Q

HOW MANY MM PER HOUR PENETRATION OF FIXATION TO THE TISSUE

A

1 MM PER HOUR

36
Q

WHAT IS THE VOLUME OF TISSUE TO FIXATIVE

A

1: 20 -25

37
Q

HOW MANY HOURS DOES TISSUE NEED TO EB EXPOSED IN FIXATIVE

A

24-48 HOURS

38
Q

WHAT WILL HAPPEN TO THE TISSUE IF IT IS EXPOSED TO THE FIXATIVE FOR MORE THAN 48 HOURS

A

TISSUE WILL HARDENED

39
Q

FACTORS AFFECTS THE FIXATION RETARTED BY:

A

SIZE AND THICKNESS OF TISSUE
BLOOD
MUCUS
FATS
COLD TEMPERATURE

40
Q

FACTORS THAT AFFECT FIXATION ENHANCED BY

A

SIZE AND THICKNESS OF THE TISSUE
AGITATION
MODERATE HEAT (37C-56C)

41
Q

PERMITS THE GENERAL MICRSCOPIC STUDY OF TISSUE STRUCTURE WITHOUT ALTERING THE STRUCTURAL PATTERN AND NORMAL INTRACELLULAR RELATIONSHIP OF TISSUE

A

MICROANATOMIC FIXATIVE

42
Q

3TYPES OF FIXATIVE ACCRODING TO ACTIONS

A

MICROANATOMIC FIXATIVE
CYTOLOGIC FIXATIVE
HISTOCHEMICAL FIXATIVE

43
Q

EXAMPLE OF MICROANATOMIC FIXATIVE

A

10% FORMOLSALINE
10% NBF
HEIDENHAIN’S SUSA
FORMOL SUBLIMATE
ZENKER’S FLUID
BOUIN’S FLUID
BRASIL SOLUTION

44
Q

USED TO PRESERVE SPECIFIC PARTS/ELEMENTS OF TISSUE

A

CYTOLOGICAL FIXATIVE

45
Q

2 TYPES OF CYTOLOGICAAL FIXATIVE

A

CYTOPLASMIC
NUCLEAR

46
Q

TYPE OF CYTOLOGICAL FIXATIVE THAT CONTAINS GLACIAL ACETIC ACID

A

NUCLEAR

47
Q

TYPE OF CYTOLOGICAL FIXATIVE THAT DOES NOT CONTAINS GLACIAL ACETIC ACID

A

CYTOPLASMIC

48
Q

EXAMPLE OF NUCLEAR CYTOLOGICAL FIXATIVE`

A

FLEMMING SOLUTION WITH GLACIAL ACETIC ACID
CARNOY’S FLUID
BOUIN SOLUTION
NEWCOMER’S FLUID
HEIDENHAIN SUSA

49
Q

EXAMPLE OF CYTOPLASMIC CYTOLOGICAL FIXATIVE

A

FLEMING’S SOLUTION WITHOUT GLACIAL ACETIC ACID
HELLY’S FLUID
REGAUD’S OR MOLLER’S FLUID, ORTH’S FLUID

50
Q

USED TO PRESERVE CHEMICAL CONSTITUENT OF THE CELL

A

HISTOCHEMICAL FIXATIVE

51
Q

EXAMPLE OF HISTOCHEMICAL FIXATIVE

A

10% FORMOL SALINE
ABSOLUTE ETHYL ALCOHOL
ACETONE
NEWCOMER’S FLUID

52
Q

2 TYPES OF FIXATIVE ACCORDING TO COMPOSITION

A

SIMPLE FIXATIVE AND COMPOUND FIXATIVE

53
Q

2 TYPES OF FIXATIVE ACCRODING TO MECHANISM OF ACTIONS

A

ADDITIVE FIXATIVE
NON-ADDITIVE FIXATIVE

54
Q

THEY CHEMICALLY ALTER THE TISSUE BY BONDING WITH IT AND ADDING THEMSELVES TO THE TISSE

A

ADDITIVE FIXATIVE

55
Q

EXAMPLEOF ADDITIVE FIXATIVE

A

MERCURIC CHLORIDE
FORMALDEHYDE
CHROMIUM TRIOXIDE
PICRIC ACID
GLUTARALDEHYDE
OSMIUM TETROXIDE
ZINC SULFATE
CHLORIDE

56
Q

ACT ONA TISSUE WITHOUT CHEMICALLY COMBINING WITH IT

A

NON-ADDITIVE FIXATIVES

57
Q

THEY ACT BY DISSOCIATING WATER FROM THE TISSUE PROETIN GROUPS CAUSING SHRINKAGE AND HARDENING OF THE TISSUE IF OVERFIXATION OCCURS

A

NON-ADDITIVE FIXATIVE

58
Q

ACT BY CREATING NETFWORK THAT ALLOWS THE SOLUTION TO READILY PENETRATE THE INTERIOR OF THE TISSUE

A

COAGULANT FIXATIVE

59
Q

CREATE A GEL THAT MAKES IT DIFFICULT TO PENETRATE BY SUBSEQUENT SOLUTIONS

A

NON-COAGULANT FIXATIVE

60
Q

SELD DIGESTION OF THE CELL BY ITS OWN FERMENT

A

AUTOLYSIS

61
Q

WHAT IS THE BEST REMEDY FOR AUTOLYSIS

A

PREVENTION

62
Q

HOW TO PREVENT AUTOLYSIS

A

MINIMIZED COLD ISCHEMIA TIME
ENSURE THE RATIO OF FIXATIVE AND TISSUE

63
Q

WHAT TECHNIQUE SHOULD BE USED IN THE SURGICAL PATHOLOGY CUTTING ROOM FOR UTERUS SPX

A

BIVALVE TECHNIQUES

64
Q

WHAT TECHNIQUE SHOULD BE USED IN THE SURGICAL PATHOLOGY CUTTING ROOM FOR GI TRACT

A

CUT THE WALL OF INTESTINE

65
Q

WHAT TECHNIQUE SHOULD BE USED IN THE SURGICAL PATHOLOGY CUTTING ROOM FOR EYE

A

INJECT FORMOL ALCOHOL BEFORE FIXATIVE

66
Q

WHAT TECHNIQUE SHOULD BE USED IN THE SURGICAL PATHOLOGY CUTTING ROOM FOR BRAIN

A

SUSPEND TO 10% BUFFERED FORMALIN FOR 3-4 WKS

67
Q

WHAT TECHNIQUE SHOULD BE USED IN THE SURGICAL PATHOLOGY CUTTING ROOM FOR HARD TISSUE- LENDRUM’S METHOD

A

SPXX WASH IN RUNNING WATER OVERNIGHT ADN PLACE TO 4% PHENOL FOR 1-3 DAYS

68
Q

EXAMPLE OF HARD TIISUE

A

CERVIX AND UTERUS

69
Q

four principle of ethics

A

autonomous
beneficence
non-maleficence
justice

70
Q

5 bioethical issues

A

Euthanasia and assisted suicide
Female genital mutilation
Abortion
Contraception
organ donor

71
Q

HB 564

A

Patient has the right the refuse diagnostic and medical treatment.

72
Q

Patient has the right to terminate his/her life upon will or with his/her physician assistance

A

HB 8148

HTMTLE (7 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions