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DAY 7 Flashcards

1
Q

PROCESS OF REPLACING THE DEHYDRATED FLUID WITH A FLUID THAT IS MISCIBLE WITH BOTH DEHYDRATING FLUID AND THE IMPREGNATING/EMBEDDING MEDIUM

A

DEALCOHOLIZATION

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2
Q

MOST COMMON CLEARING AGENT

A

XYLENE

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3
Q

CLEARING AGENT THAT IS USED FOR SUBSTITUTE FOR XYLENE

A

TOLUENE

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4
Q

CLEARING AGENT THAT IS TOXIC TO LIVER AFTER PROLONGED INHALATION AND IT DOES NOT MAKE THETISSUE TRANSPARENT

A

CHLOROFORM

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5
Q

CLEARING AGENT THAT IS TOXIC TO SMOOTH MUSCLE CNS

A

CEDAR WOOD OIL

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6
Q

CLEARING AGENT THAT IS CARCINOGENIC, AND CAN CAUSE APLASTIC ANEMIA

A

BENZENE

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7
Q

CLEARING AGENT THAT IS PARTIAL DEHYDRANT OF ENBRYO, INSECT, VERY DELICATE SPECIMEN

A

ANILINE

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8
Q

PROCESS OF REPLACING THE CLEARING AGENT WITH INFLITRATING MEDIUM

A

INFILTRATION

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9
Q

SIMPLEST MOST COMMON AND BEST INFILTRATING EMBEDDING MEDIUM
NOT RECOMMENDED FOR FATTY TISSUE AND FROZEN SECTION

A

PARAFFIN

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10
Q

TEMPERATURE OF PARAFIN OVEN

A

55-60C

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11
Q

TO REMOVE EXCESS WATER

A

HEAT THE WAC TO 100-105C

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12
Q

SUBSTITUTE FOR PARAFFIN MIXTURE OF HIGHLY PURIFIED AND SYNTHETIC PLASTIC POLYMERS

A

PARAPLAST

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13
Q

SUBSTITUTE FOR PARAFFIN LESS BRITTLE AND LESS COMPRESSIBLE THAN PARAPLAST

A

EMBEDDOL

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14
Q

SUBSTITUTE FOR PARAFFIN RECOMMENDED FOR EMBEDDING EYES

A

BIOLOID

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15
Q

A PRODUCT OF PARAFFIN, CONTAINING RUBBER WITH THE SAME PROPERTY AS PARAPLAST

A

TISSUE MAT

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16
Q

SUBSTITUTE FOR PARAFFIN THAT HARDER THAN PARAFFIN
NOT SOLUBLE IN WATER
SOLUBLE IN 95ETOH AND OTHER CLEARING AGENT

A

ESTER WAX

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17
Q

MOST COMMOLY USED WAX AND MOSTLY POLYETHYLENE GLYCOLS

A

WATER SOLUBLE WAXES

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18
Q

SUITABLE FOR SPECIMENS WITH ALARGE HOLLOW CAVITIES AND DENSE TISSUES , LARGE TISSUE SECTION OF THE WHOLE EMBRYO

A

CELLOIDIN

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19
Q

METHOD OF CELLOIDIN THAT IS RECOMMENDED FOR BONES, TEETH, LARGE BRAINS AND WHOLE ORGAN

A

WET

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20
Q

METHOD OF CELLOIDIN PREFFERED FOR PROCESSING WHOLE EYE SECTION

A

DRY

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21
Q

SOLUBLE IN EQUAL CONCENTRATION OF ETHER AND ALCOHOL WITH LOWER VISCOSITY,A LLOWING IT TO BE USED IN HIGHER CONC. AND STILL PENETRATE TISSUE RAPIDLY

A

LOW VISCOSITY NITROCELLULOSE

22
Q

USED WHEN DHYDRATION IS TO BE AVOIDED
USE FOR HISTOCHEM AND ENZYME STUDIES
EMBEDDING MEIDUM FOR DELICATE SPECIMEN AND FROZEN SECTION
DOES NOT REQUIRE DEHYDRATION AND CLEARING

23
Q

CLASSIFIED INTO EPOXY, POLYSTER AND ACRYLIC

A

PLASTIC/RESIN

24
Q

PROCESS BY WHICH THE IMPREGNATED TISSUE IS PLACED INTO A PRECISELY ARRANGED POSITION IN MOLD

A

CASTING OR BLOCKING

25
PROCESS BY WHICH TISSUE IS ARRANGED IN PRECISE POSITION IN THE MOLD DURING EMBEDDING ON THE MICROTOME BEFORE CUTTING AND ON THE SLIDE BEFORE STAINING
ORIENTATION
26
TEMPERATURE OF MELTED PARAFFIN USED FOR EMBEDDING
5-10C ABOVE ITS MELTING POINT
27
TEMPERATURE TO SOLIDIFY EMBEDDED TIISUE COLLED RAPIDLY IN A REF
5C
28
A STEP THAT GOES HAND IN HAND WITH EMBEDDING IF INDIVIDUAL MODE IS USED BECAUSE BLOCKS ARE PRODUCED AFTER SOLIDIFICATION
BLOCKING
29
4 TYPES OF EMBEDDING MOLD ACCORDING TO ITS COMPOSITION
PLASTIC METAL MADE OF PAPER GLASS2
30
2 TYPES OF MOLD ACCORDING TO USE
DISPOSABLE NON-DISPOSABLE
31
PROCESS OF REMOVING EXCESS WAX AFTER EMBEDDING EXCESS WAX IS CUT OFF FROM THE BLOCK TO EXPOSE THE TISSUE SURFACE IN PREPARATION FOR ACTUAL CUTTING
TRIMMING
32
THE PROCESS BY WHICH A PROCESSED TISSUE CUT INTO UNIFORMLY SLICES TO FACILITATES STUDIES UNDER THE MICROSCOPE
MICROTOMY
33
HOW MANY MM DOES THE TISSUE NEED IN FROZEN SECTION?
10-15MM
34
HOW MANY MM DOES THE TISSUE NEED IN ROUTINE HISTOLOGIC PROCEDURE>
4-6MM
35
HOW MANY MM DOES THE TISSUE NEED IN ELECTRON MICROSCOPY?
0.5MM
36
TYPE OF MICROTOME THAT IS SIMPLEST AMONG TEH MICROTOMES
ROCKING MICROTOME
37
WHO IS THE INVENTOR OF ROCKING MICROTOME
PALDWELL TREFALL
38
MOST COMMON TYPE OF MICROTOME USED TODAY ESPECIALLY FOR PARAFILM EMBEDDED TISSUE
ROTARY/MINOT MICROTOME
39
WHO IS THE INVENTOR OF ROTARY MICROTOME
MINOT
40
MOST DANGEROUS TYPE OF MICROTOME
SLIDING MICROTOME
41
WHO IS THE INVENTOR OF SLIDING MICROTOME
ADAMS
42
TYPE OF SLIDING MICROTOME BLOCK HOLDER: MOVING KNIFE: STATIONARY
BASE-SLEDGE
43
TYPE OF SLIDING MICROTOME BLOCK HOLDER: STATIONARY KNIFE: MOVING
STANDARD SLDING MICROTOME
44
MICROTOME USED FOR UNFIXED, UNFORZEN SPECIMEN SECTIONING FOR ENZYME
VIBROTOME
45
MICROTOME USED FOR CUTTING SECTIONS FOR ELECTRON MICROSCOPY
ULTRATHIN MICROTOME
46
WHAT KNIFE IS USED IN ULTRATHIN MICROTOME?
DIAMOND KNIVES OR BROKEN PLATE GLASS
47
MICROTOME THAT IS INVENTED BY QUECKETT
FREEZING MICROTOME
48
CLEARANCE ANGLE FOR FREEZING MICROTOME
0-15 DEGREE
49
OPTIMUM CUTTING ANGLE FOR FREEZING MICROTOME
15 DEGREE
50
BEVEL ANGLE IN FREEZING MICROTOME
27-32 DEGREE
51
WHAT IS THE TEMP OF WATER BATH?
45-50C

HTMTLE (7 decks)

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