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HTMTLE > DAY 7 > Flashcards

DAY 7 Flashcards

1
Q

PROCESS OF REPLACING THE DEHYDRATED FLUID WITH A FLUID THAT IS MISCIBLE WITH BOTH DEHYDRATING FLUID AND THE IMPREGNATING/EMBEDDING MEDIUM

A

DEALCOHOLIZATION

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2
Q

MOST COMMON CLEARING AGENT

A

XYLENE

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3
Q

CLEARING AGENT THAT IS USED FOR SUBSTITUTE FOR XYLENE

A

TOLUENE

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4
Q

CLEARING AGENT THAT IS TOXIC TO LIVER AFTER PROLONGED INHALATION AND IT DOES NOT MAKE THETISSUE TRANSPARENT

A

CHLOROFORM

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5
Q

CLEARING AGENT THAT IS TOXIC TO SMOOTH MUSCLE CNS

A

CEDAR WOOD OIL

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6
Q

CLEARING AGENT THAT IS CARCINOGENIC, AND CAN CAUSE APLASTIC ANEMIA

A

BENZENE

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7
Q

CLEARING AGENT THAT IS PARTIAL DEHYDRANT OF ENBRYO, INSECT, VERY DELICATE SPECIMEN

A

ANILINE

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8
Q

PROCESS OF REPLACING THE CLEARING AGENT WITH INFLITRATING MEDIUM

A

INFILTRATION

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9
Q

SIMPLEST MOST COMMON AND BEST INFILTRATING EMBEDDING MEDIUM
NOT RECOMMENDED FOR FATTY TISSUE AND FROZEN SECTION

A

PARAFFIN

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10
Q

TEMPERATURE OF PARAFIN OVEN

A

55-60C

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11
Q

TO REMOVE EXCESS WATER

A

HEAT THE WAC TO 100-105C

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12
Q

SUBSTITUTE FOR PARAFFIN MIXTURE OF HIGHLY PURIFIED AND SYNTHETIC PLASTIC POLYMERS

A

PARAPLAST

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13
Q

SUBSTITUTE FOR PARAFFIN LESS BRITTLE AND LESS COMPRESSIBLE THAN PARAPLAST

A

EMBEDDOL

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14
Q

SUBSTITUTE FOR PARAFFIN RECOMMENDED FOR EMBEDDING EYES

A

BIOLOID

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15
Q

A PRODUCT OF PARAFFIN, CONTAINING RUBBER WITH THE SAME PROPERTY AS PARAPLAST

A

TISSUE MAT

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16
Q

SUBSTITUTE FOR PARAFFIN THAT HARDER THAN PARAFFIN
NOT SOLUBLE IN WATER
SOLUBLE IN 95ETOH AND OTHER CLEARING AGENT

A

ESTER WAX

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17
Q

MOST COMMOLY USED WAX AND MOSTLY POLYETHYLENE GLYCOLS

A

WATER SOLUBLE WAXES

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18
Q

SUITABLE FOR SPECIMENS WITH ALARGE HOLLOW CAVITIES AND DENSE TISSUES , LARGE TISSUE SECTION OF THE WHOLE EMBRYO

A

CELLOIDIN

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19
Q

METHOD OF CELLOIDIN THAT IS RECOMMENDED FOR BONES, TEETH, LARGE BRAINS AND WHOLE ORGAN

A

WET

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20
Q

METHOD OF CELLOIDIN PREFFERED FOR PROCESSING WHOLE EYE SECTION

A

DRY

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21
Q

SOLUBLE IN EQUAL CONCENTRATION OF ETHER AND ALCOHOL WITH LOWER VISCOSITY,A LLOWING IT TO BE USED IN HIGHER CONC. AND STILL PENETRATE TISSUE RAPIDLY

A

LOW VISCOSITY NITROCELLULOSE

22
Q

USED WHEN DHYDRATION IS TO BE AVOIDED
USE FOR HISTOCHEM AND ENZYME STUDIES
EMBEDDING MEIDUM FOR DELICATE SPECIMEN AND FROZEN SECTION
DOES NOT REQUIRE DEHYDRATION AND CLEARING

A

GELATIN

23
Q

CLASSIFIED INTO EPOXY, POLYSTER AND ACRYLIC

A

PLASTIC/RESIN

24
Q

PROCESS BY WHICH THE IMPREGNATED TISSUE IS PLACED INTO A PRECISELY ARRANGED POSITION IN MOLD

A

CASTING OR BLOCKING

25
Q

PROCESS BY WHICH TISSUE IS ARRANGED IN PRECISE POSITION IN THE MOLD DURING EMBEDDING ON THE MICROTOME BEFORE CUTTING AND ON THE SLIDE BEFORE STAINING

A

ORIENTATION

26
Q

TEMPERATURE OF MELTED PARAFFIN USED FOR EMBEDDING

A

5-10C ABOVE ITS MELTING POINT

27
Q

TEMPERATURE TO SOLIDIFY EMBEDDED TIISUE COLLED RAPIDLY IN A REF

A

5C

28
Q

A STEP THAT GOES HAND IN HAND WITH EMBEDDING IF INDIVIDUAL MODE IS USED BECAUSE BLOCKS ARE PRODUCED AFTER SOLIDIFICATION

A

BLOCKING

29
Q

4 TYPES OF EMBEDDING MOLD ACCORDING TO ITS COMPOSITION

A

PLASTIC
METAL
MADE OF PAPER
GLASS2

30
Q

2 TYPES OF MOLD ACCORDING TO USE

A

DISPOSABLE
NON-DISPOSABLE

31
Q

PROCESS OF REMOVING EXCESS WAX AFTER EMBEDDING
EXCESS WAX IS CUT OFF FROM THE BLOCK TO EXPOSE THE TISSUE SURFACE IN PREPARATION FOR ACTUAL CUTTING

A

TRIMMING

32
Q

THE PROCESS BY WHICH A PROCESSED TISSUE CUT INTO UNIFORMLY SLICES TO FACILITATES STUDIES UNDER THE MICROSCOPE

A

MICROTOMY

33
Q

HOW MANY MM DOES THE TISSUE NEED IN FROZEN SECTION?

A

10-15MM

34
Q

HOW MANY MM DOES THE TISSUE NEED IN ROUTINE HISTOLOGIC PROCEDURE>

A

4-6MM

35
Q

HOW MANY MM DOES THE TISSUE NEED IN ELECTRON MICROSCOPY?

A

0.5MM

36
Q

TYPE OF MICROTOME THAT IS SIMPLEST AMONG TEH MICROTOMES

A

ROCKING MICROTOME

37
Q

WHO IS THE INVENTOR OF ROCKING MICROTOME

A

PALDWELL TREFALL

38
Q

MOST COMMON TYPE OF MICROTOME USED TODAY ESPECIALLY FOR PARAFILM EMBEDDED TISSUE

A

ROTARY/MINOT MICROTOME

39
Q

WHO IS THE INVENTOR OF ROTARY MICROTOME

A

MINOT

40
Q

MOST DANGEROUS TYPE OF MICROTOME

A

SLIDING MICROTOME

41
Q

WHO IS THE INVENTOR OF SLIDING MICROTOME

A

ADAMS

42
Q

TYPE OF SLIDING MICROTOME
BLOCK HOLDER: MOVING
KNIFE: STATIONARY

A

BASE-SLEDGE

43
Q

TYPE OF SLIDING MICROTOME
BLOCK HOLDER: STATIONARY
KNIFE: MOVING

A

STANDARD SLDING MICROTOME

44
Q

MICROTOME USED FOR UNFIXED, UNFORZEN SPECIMEN SECTIONING FOR ENZYME

A

VIBROTOME

45
Q

MICROTOME USED FOR CUTTING SECTIONS FOR ELECTRON MICROSCOPY

A

ULTRATHIN MICROTOME

46
Q

WHAT KNIFE IS USED IN ULTRATHIN MICROTOME?

A

DIAMOND KNIVES OR BROKEN PLATE GLASS

47
Q

MICROTOME THAT IS INVENTED BY QUECKETT

A

FREEZING MICROTOME

48
Q

CLEARANCE ANGLE FOR FREEZING MICROTOME

A

0-15 DEGREE

49
Q

OPTIMUM CUTTING ANGLE FOR FREEZING MICROTOME

A

15 DEGREE

50
Q

BEVEL ANGLE IN FREEZING MICROTOME

A

27-32 DEGREE

51
Q

WHAT IS THE TEMP OF WATER BATH?

A

45-50C

HTMTLE (7 decks)

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