DAY 7 Flashcards
PROCESS OF REPLACING THE DEHYDRATED FLUID WITH A FLUID THAT IS MISCIBLE WITH BOTH DEHYDRATING FLUID AND THE IMPREGNATING/EMBEDDING MEDIUM
DEALCOHOLIZATION
MOST COMMON CLEARING AGENT
XYLENE
CLEARING AGENT THAT IS USED FOR SUBSTITUTE FOR XYLENE
TOLUENE
CLEARING AGENT THAT IS TOXIC TO LIVER AFTER PROLONGED INHALATION AND IT DOES NOT MAKE THETISSUE TRANSPARENT
CHLOROFORM
CLEARING AGENT THAT IS TOXIC TO SMOOTH MUSCLE CNS
CEDAR WOOD OIL
CLEARING AGENT THAT IS CARCINOGENIC, AND CAN CAUSE APLASTIC ANEMIA
BENZENE
CLEARING AGENT THAT IS PARTIAL DEHYDRANT OF ENBRYO, INSECT, VERY DELICATE SPECIMEN
ANILINE
PROCESS OF REPLACING THE CLEARING AGENT WITH INFLITRATING MEDIUM
INFILTRATION
SIMPLEST MOST COMMON AND BEST INFILTRATING EMBEDDING MEDIUM
NOT RECOMMENDED FOR FATTY TISSUE AND FROZEN SECTION
PARAFFIN
TEMPERATURE OF PARAFIN OVEN
55-60C
TO REMOVE EXCESS WATER
HEAT THE WAC TO 100-105C
SUBSTITUTE FOR PARAFFIN MIXTURE OF HIGHLY PURIFIED AND SYNTHETIC PLASTIC POLYMERS
PARAPLAST
SUBSTITUTE FOR PARAFFIN LESS BRITTLE AND LESS COMPRESSIBLE THAN PARAPLAST
EMBEDDOL
SUBSTITUTE FOR PARAFFIN RECOMMENDED FOR EMBEDDING EYES
BIOLOID
A PRODUCT OF PARAFFIN, CONTAINING RUBBER WITH THE SAME PROPERTY AS PARAPLAST
TISSUE MAT
SUBSTITUTE FOR PARAFFIN THAT HARDER THAN PARAFFIN
NOT SOLUBLE IN WATER
SOLUBLE IN 95ETOH AND OTHER CLEARING AGENT
ESTER WAX
MOST COMMOLY USED WAX AND MOSTLY POLYETHYLENE GLYCOLS
WATER SOLUBLE WAXES
SUITABLE FOR SPECIMENS WITH ALARGE HOLLOW CAVITIES AND DENSE TISSUES , LARGE TISSUE SECTION OF THE WHOLE EMBRYO
CELLOIDIN
METHOD OF CELLOIDIN THAT IS RECOMMENDED FOR BONES, TEETH, LARGE BRAINS AND WHOLE ORGAN
WET
METHOD OF CELLOIDIN PREFFERED FOR PROCESSING WHOLE EYE SECTION
DRY
SOLUBLE IN EQUAL CONCENTRATION OF ETHER AND ALCOHOL WITH LOWER VISCOSITY,A LLOWING IT TO BE USED IN HIGHER CONC. AND STILL PENETRATE TISSUE RAPIDLY
LOW VISCOSITY NITROCELLULOSE
USED WHEN DHYDRATION IS TO BE AVOIDED
USE FOR HISTOCHEM AND ENZYME STUDIES
EMBEDDING MEIDUM FOR DELICATE SPECIMEN AND FROZEN SECTION
DOES NOT REQUIRE DEHYDRATION AND CLEARING
GELATIN
CLASSIFIED INTO EPOXY, POLYSTER AND ACRYLIC
PLASTIC/RESIN
PROCESS BY WHICH THE IMPREGNATED TISSUE IS PLACED INTO A PRECISELY ARRANGED POSITION IN MOLD
CASTING OR BLOCKING
PROCESS BY WHICH TISSUE IS ARRANGED IN PRECISE POSITION IN THE MOLD DURING EMBEDDING ON THE MICROTOME BEFORE CUTTING AND ON THE SLIDE BEFORE STAINING
ORIENTATION
TEMPERATURE OF MELTED PARAFFIN USED FOR EMBEDDING
5-10C ABOVE ITS MELTING POINT
TEMPERATURE TO SOLIDIFY EMBEDDED TIISUE COLLED RAPIDLY IN A REF
5C
A STEP THAT GOES HAND IN HAND WITH EMBEDDING IF INDIVIDUAL MODE IS USED BECAUSE BLOCKS ARE PRODUCED AFTER SOLIDIFICATION
BLOCKING
4 TYPES OF EMBEDDING MOLD ACCORDING TO ITS COMPOSITION
PLASTIC
METAL
MADE OF PAPER
GLASS2
2 TYPES OF MOLD ACCORDING TO USE
DISPOSABLE
NON-DISPOSABLE
PROCESS OF REMOVING EXCESS WAX AFTER EMBEDDING
EXCESS WAX IS CUT OFF FROM THE BLOCK TO EXPOSE THE TISSUE SURFACE IN PREPARATION FOR ACTUAL CUTTING
TRIMMING
THE PROCESS BY WHICH A PROCESSED TISSUE CUT INTO UNIFORMLY SLICES TO FACILITATES STUDIES UNDER THE MICROSCOPE
MICROTOMY
HOW MANY MM DOES THE TISSUE NEED IN FROZEN SECTION?
10-15MM
HOW MANY MM DOES THE TISSUE NEED IN ROUTINE HISTOLOGIC PROCEDURE>
4-6MM
HOW MANY MM DOES THE TISSUE NEED IN ELECTRON MICROSCOPY?
0.5MM
TYPE OF MICROTOME THAT IS SIMPLEST AMONG TEH MICROTOMES
ROCKING MICROTOME
WHO IS THE INVENTOR OF ROCKING MICROTOME
PALDWELL TREFALL
MOST COMMON TYPE OF MICROTOME USED TODAY ESPECIALLY FOR PARAFILM EMBEDDED TISSUE
ROTARY/MINOT MICROTOME
WHO IS THE INVENTOR OF ROTARY MICROTOME
MINOT
MOST DANGEROUS TYPE OF MICROTOME
SLIDING MICROTOME
WHO IS THE INVENTOR OF SLIDING MICROTOME
ADAMS
TYPE OF SLIDING MICROTOME
BLOCK HOLDER: MOVING
KNIFE: STATIONARY
BASE-SLEDGE
TYPE OF SLIDING MICROTOME
BLOCK HOLDER: STATIONARY
KNIFE: MOVING
STANDARD SLDING MICROTOME
MICROTOME USED FOR UNFIXED, UNFORZEN SPECIMEN SECTIONING FOR ENZYME
VIBROTOME
MICROTOME USED FOR CUTTING SECTIONS FOR ELECTRON MICROSCOPY
ULTRATHIN MICROTOME
WHAT KNIFE IS USED IN ULTRATHIN MICROTOME?
DIAMOND KNIVES OR BROKEN PLATE GLASS
MICROTOME THAT IS INVENTED BY QUECKETT
FREEZING MICROTOME
CLEARANCE ANGLE FOR FREEZING MICROTOME
0-15 DEGREE
OPTIMUM CUTTING ANGLE FOR FREEZING MICROTOME
15 DEGREE
BEVEL ANGLE IN FREEZING MICROTOME
27-32 DEGREE
WHAT IS THE TEMP OF WATER BATH?
45-50C
