HTLV1 and Prions

Question 1

HTLV1:

Answer 1

delta retrovirus, oncogenic

Question 2

HTLV1:

Answer 2

Structure – 2 ssRNA, icosahedral, enveloped

Epidemiology – Caribbean, Japan, West Africa

Question 3

HTLV1:

Answer 3

Replication – reverse transcriptase → genome integration → replication → virus stays in the cells, PIC transferred to adjacent cell

Question 4

HTLV1: Adult T cell leukemia

Answer 4

– floral nuclei T cells, skin lesions – acute, chronic, smoldering, lymphoma

Question 5

HTLV1: Tropical spastic paraparesis/myelopathy (TSP/HAM)

Answer 5

• ↑ infected T cells, cytokines and CD8 → Tax → demyelination

Question 6

Tax protein

Answer 6

– gene expression, inhibition of apoptosis, activates growth factors, inhibits DNA repair
CD8 T cell target → ↓ proliferation, Tax protein loss, HBZ expression → floral nuclei, disease

Question 7

HBZ

Answer 7

– stimulates T cells production (E2F1), down regulation of Tax

Question 8

Prions:

Answer 8

case transmissible spongiform encephalopathies

Question 9

Prions: Histo

Answer 9

– vacuolation of neurons, amyloid aggregates, atrophy w/ inflammation

Question 10

Bovine spongiform encephalopathy

Answer 10

– aka Mad Cow, variant Creutzfeldt-Jacob disease

Question 11

Scrapie protein

Answer 11

– β sheet dominant, insoluble, resistant to proteinase K degradation, recruits other normal proteins