HSCT

Question 1

Stem Cell Source Selection (Matched Related or Unrelated Donors) goal ?

Answer 1

The preferred CD34+ dose is at least 4-5 × 10⁶ CD34+ cells/kg.

Question 2

Stem Cell Source Selection (Haploidentical Donors) goal?

Answer 2

For T-cell depleted graft, the preferred CD34+ dose is 10-20 × 10⁶ cells/kg, with minimal T-cell content (CD3+ <1 × 10⁵ cells).

When using post-transplant cyclophosphamide, a higher CD34+ count is preferred.

Question 3

Stem Cell Source Selection (Cord Blood) goal?

Answer 3

The standard target is ≥4 × 10⁷ total nucleated cells (TNC)/kg.

Question 4

Strongest risk for relaspe after CAR-T

Answer 4

NGS-MRD positivity at 3 months, is associated with essentially a 100% risk of relapse. This is because it can detect both CD19+ and CD19- relapses.

Question 5

Collection dose for auto

Answer 5

Goal precollection is 20 CD34+ cells/µL if it is < 20 and on GCSF add plerixafor in the evening.